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目的探讨海洋胶原肽对窒息仔鼠近期及远期神经认知功能影响。方法 70只健康成年SD大鼠交配受孕,孕鼠于临产前1 d行延迟剖宫产术,建立新生仔鼠窒息模型;窒息仔鼠随机分为窒息组和海洋胶原肽低(0.225%)、中(0.45%)、高(1.35%)剂量组,正常分娩仔鼠为对照组;观察仔鼠出生后生理发育和神经反射达标情况,并在断乳时和3月龄分别进行Morris水迷宫实验。结果窒息组和3个MCPs干预组生理和神经反射指标达标时间均长于对照组(P<0.05);Morris水迷宫实验结果显示,断乳时窒息组和3个MCPs干预组逃避潜伏期较对照组延长(P<0.05);对照组、窒息组、低、中、高MCPs组穿越平台次数分别为(7.00±1.706)、(3.92±1.505)、(4.33±1.497)、(4.50±1.931)、(4.67±1.371),差异有统计学意义(P<0.05);3月龄时,与窒息组比较,3个MCPs干预组大鼠逃避潜伏期明显缩短(P<0.01)、穿越平台次数明显增多(P<0.05)。结论一定剂量的海洋胶原肽能够改善窒息仔鼠远期学习记忆能力。
Objective To investigate the effect of marine collagen peptide on neurocognitive function of asphyxiated neonatal rats in the near and long term. Methods Seventy healthy adult SD rats were mated to conceive. Pregnant rats were delayed cesarean section 1 d before labor to establish neonatal asphyxia model. Asphyxia rats were randomly divided into asphyxia group and marine collagen peptide (0.225%), (0.45%), high (1.35%) dose group, the normal delivery offspring as a control group; observe the physiological development and neurological reflex compliance of the offspring after birth, and at the weaning and 3 months of age, Morris water maze test . Results The target time of physiological and neurological reflexes in asphyxial group and three MCPs intervention groups were longer than those in control group (P <0.05). The results of Morris water maze test showed that the escape latency of asphyxia group and three MCPs intervention groups was longer than that of control group (P <0.05). The number of crossing platform of control, asphyxia group, low, middle and high MCPs group were (7.00 ± 1.706), (3.92 ± 1.505), (4.33 ± 1.497), (4.50 ± 1.931) ± 1.371) (P <0.05). Compared with asphyxia group, the escape latency of three MCPs intervention groups was significantly shorter (P <0.01) and the number of crossing platform significantly increased at 3 months (P < 0.05). Conclusion A certain dose of marine collagen peptide can improve long-term learning and memory ability of asphyxiated offspring.