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目的探讨钙囊素(S100A11)在大肠癌组织中的表达及其临床意义。方法荧光定量PCR法检测15例新鲜大肠癌及其癌旁组织中S100A11mRNA表达,免疫组化法检测59例大肠癌及26例癌旁组织中S100A11蛋白表达;分析其与临床病理特征及预后的关系。结果大肠癌组织中S100A11mRNA表达量[0.22846(0.02019-2.88786)]和蛋白表达阳性率(88.5%)均高于相应的癌旁组织[0.05711(0.01252-2.08493)和34.6%](P<0.05)。S100A11蛋白表达强度与大肠癌的分化程度、肿瘤浸润深度、淋巴转移、Dukes分期和3年生存期密切相关(P<0.05)。肿瘤分化程度、浸润深度、有无淋巴结及远处转移、Dukes分期及S100A11阳性与大肠癌患者生存期密切相关(P<0.05);S100A11阳性与大肠癌患者总生存率的降低显著相关(P<0.01)。结论 S100A11可作为大肠癌独立的预后指标。
Objective To investigate the expression of calcineurin (S100A11) in colorectal carcinoma and its clinical significance. Methods The expression of S100A11 mRNA in 15 cases of fresh colorectal carcinoma and its adjacent tissues was detected by real-time fluorescence quantitative PCR. The expressions of S100A11 protein in 59 cases of colorectal cancer and 26 cases of paracancerous tissues were detected by immunohistochemistry. The relationship between S100A11 expression and the clinicopathological features and prognosis . Results The expression of S100A11 mRNA in colorectal cancer tissues was significantly higher than that in corresponding adjacent tissues [0.22846 (0.02019-2.88786) and 88.5% (P <0.05). The expression of S100A11 protein was closely related to the differentiation of colorectal cancer, the depth of tumor invasion, lymphatic metastasis, Dukes stage and 3-year survival (P <0.05). The degree of tumor differentiation, depth of invasion, lymph node metastasis and distant metastasis, Dukes staging and S100A11 positive were closely related to the survival of patients with colorectal cancer (P <0.05). The positive rate of S100A11 was significantly correlated with the decrease of overall survival rate of patients with colorectal cancer (P < 0.01). Conclusion S100A11 can be used as an independent prognostic indicator for colorectal cancer.