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目的:比较RT-PCR法与RD细胞病毒分离法两种检测方法对监测EV71病毒结果的影响。方法:将151份标本分别经RT-PCR法与RD细胞病毒分离法检测出的阳性标本按不同分类及标本总阳性率进行数据统计,比较两种检验方法间的差异。结果:RT-PCR法与RD细胞病毒分离法检测出的阳性标本按不同分类统计比较后,两者无明显差异,无统计学意义(P>0.05);两种方法检测出的标本总阳性率RD细胞病毒分离法为38.41%,而RT-PCR法为26.49%,差异有统计学意义(2χ=4.89,P(0.05)。结论:RT-PCR法检测结果可以作为手足口EV71病毒监测结果进行分类数据统计分析,而标本监测年度总阳性率应以RD细胞病毒分离检测结果统计。
Objective: To compare the effects of RT-PCR and RD virus isolation on the detection of EV71 virus. Methods: 151 samples were detected by RT-PCR and RD virus isolation of positive specimens according to different categories and the total positive rate of specimens statistics, the difference between the two test methods. Results: There was no significant difference between the positive samples detected by RT-PCR and RD virus isolation methods according to different categories (P> 0.05). The positive rate of the two samples The RD virus isolation rate was 38.41%, while RT-PCR was 26.49%, the difference was statistically significant (2χ = 4.89, P (0.05) .Conclusion: RT-PCR results can be used as monitoring results of hand, foot and mouth EV71 virus Statistical analysis of classification data, and the total annual positive rate of specimen monitoring should be based on RD virus isolation test results statistics.