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目的观察大蒜素对人肝癌细胞HepG2的抑制和诱导细胞凋亡作用。方法用不同浓度大蒜素处理对数生长期的人肝癌细胞HepG2。比色还原法检查4、8、16 h时HepG2细胞生长的抑制率;Hoechest荧光染色观察8 h时细胞形态的变化;JC-1检测40 mg/L大蒜素作用4 h后细胞线粒体跨膜电位的变化;免疫组化法检测经浓度为40 mg/L大蒜素作用4 h后其对细胞色素C的影响。结果与无药阴性对照组比较,大蒜素能明显抑制HepG2细胞的增殖,且抑制率随浓度增加和作用时间延长而升高(P<0.01)。Hoechst 33258荧光染色观察到细胞凋亡形态学特征。结论 大蒜素对人肝癌细胞株HepG2有明显的生长抑制作用,诱导人肝癌细胞株HepG2的凋亡。这可能与其通过线粒体途径引起线粒体跨膜电位下降与细胞色素C的释放有关。
Objective To observe the inhibitory effect of allicin on human hepatoma HepG2 cells and induce apoptosis. Methods Human liver cancer HepG2 cells were treated with different concentrations of allicin. The inhibitory rate of HepG2 cell growth was detected by colorimetric reduction assay at 4, 8 and 16 h, the cell morphology was observed by Hoechest fluorescent staining at 8 h, the mitochondrial transmembrane potential The changes of cytochrome C were detected by immunohistochemistry after allicin was treated with 40 mg / L allicin for 4 h. Results Allicin significantly inhibited the proliferation of HepG2 cells compared with no drug-negative control group, and the inhibition rate increased with the increase of concentration and time (P <0.01). Hoechst 33258 fluorescence staining observed apoptotic morphological features. Conclusion Allicin can significantly inhibit the growth of human hepatocellular carcinoma cell line HepG2 and induce the apoptosis of human hepatocellular carcinoma cell line HepG2. This may be related to its mitochondrial pathway caused by mitochondrial transmembrane potential drop and the release of cytochrome C.