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目的:微流控芯片作为一项全新的辅助生殖技术,尝试基于该芯片设计出一种模拟体内输卵管受精状态的分选精子的方式。方法:按功能构建微流控芯片,通过研究通道中不同部分的精子动力学及流体运动来实现精子分选。整个过程近似模拟体内输卵管受精状态。结果:通过对11例精液标本的微流控芯片分选,在通道入口,(A级+B级)%精子从37%提升到80%,同时,在通道出口,(A级+B级)%精子从37%降低到16%。结论:这种全新的微流控分选精子的方法不仅降低了分选过程中对精子的损伤,提高了分选的效率,同时实现了微量分选,从而为微量精液受精提供了可能。而且,这种方法充分模拟了体内输卵管精子运动的过程,为进一步的研究奠定了基础。
Objective: Microfluidic chip as a new assisted reproductive technology, try to design based on the chip to simulate the status of tubal insemination in vivo sorting sperm way. Methods: The microfluidic chip was constructed according to the function, and the sperm sorting was realized by studying the sperm dynamics and fluid movement in different parts of the channel. The whole process approximately simulates the status of tubal fertilization in vivo. Results: According to the microfluidic chip sorting of 11 semen specimens, the percentage of spermatozoa increased from 37% to 80% at the entrance of the channel (A + B), and at the exit of the channel (A + B) % Sperm decreased from 37% to 16%. Conclusion: This new method of microfluidic sorting of sperm not only reduces the damage of sperm in the process of sorting, improves the efficiency of sorting, but also realizes the micro-sorting, which provides the possibility of micro-semen fertilization. Moreover, this method fully simulated the process of tubal sperm motility in vivo, which laid the foundation for further research.