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目的研究法尼酯X受体(farnesoid X receptor,FXR)对成纤维细胞生长因子21(fibroblast growth factor,FGF21)表达的影响。方法用FXR特异性激动剂终浓度为0、50、100μmol/L的CDCA和0、1、5μmol/L的GW4064分别处理人肝癌细胞株HepG2和人胚胎肝细胞L02细胞后,用逆转录-聚合酶链法(RT-PCR)检测FXR特异性靶基因小异源二聚体配体(small heterodimer parterner,SHP)和FGF21 mRNA的表达变化,再用Western blot法检测FGF21蛋白表达水平的变化。结果用FXR特异性激动剂CDCA和GW4064刺激后,分别比较HepG2和L02细胞SHP与β-actin mRNART-PCR产物光密度比值,比值均明显增高(P<0.05),反应HepG2和L02细胞内SHP mRNA水平均明显升高,表明FXR在2种细胞中被激活;分别比较HepG2和L02细胞FGF21与β-actin RT-PCR产物光密度比值以及FGF21与β-actin蛋白光密度比值,比值均明显升高(P<0.05),表明FGF21 mRNA和蛋白水平均明显升高。结论激活的FXR可以上调FGF21的表达。
Objective To investigate the effect of farnesoid X receptor (FXR) on the expression of fibroblast growth factor (FGF21). Methods Human hepatocellular carcinoma cell line HepG2 and human embryonic liver cell line L02 were treated with 0, 50 and 100 μmol / L of CDCA and 0, 1 and 5 μmol / L of GW4064 respectively. The mRNA expression of FGF21 and small heterodimer parterner (FXR) was detected by RT-PCR. The expression of FGF21 protein was detected by Western blot. Results After stimulated with FXR specific agonists CDCA and GW4064, the ratios of SHP to β-actin mRNART-PCR products in HepG2 and L02 cells were significantly increased (P <0.05) The levels of FGF21 and β-actin RT-PCR products in HepG2 and L02 cells were significantly higher than those in the other two groups (P <0.05), indicating that FGF21 mRNA and protein levels were significantly increased. Conclusion Activated FXR can up-regulate the expression of FGF21.