论文部分内容阅读
目的 研究慢性无症状HBV感染者HBV前C区 1896位G -A突变发生情况及肝组织炎症活动度、纤维化分级 ,探讨其临床意义。方法 采用苏木精依红 (HE)及网状纤维 (Gorden -Sweet法 )和胶原纤维 (GV)法染色研究肝组织炎症活动度、纤维分级 ,采用PCR -RFLP法HBV前C区 1896位G -A突变。结果 2 5例慢性无症状HBV感染者中除 3例 (12 % )无慢性肝炎的病理变化外 ,其余病例均有慢性肝炎的病理变化 ,其中 3例已出现明显的汇管区周围纤维化 ,纤维间隔形成 ;与 2 4例慢性乙型肝炎患者进行临床病理对照研究发现 ,慢性无症状HBV感染者的肝组织炎症活动度及纤维化分级均低于慢性肝炎病人 ,且随着病人临床表现的加重 ,病人肝组织的炎症活动度及纤维化分级也增加。HBV前C区 1896位G -A突变在慢性无症状HBV感染者和慢性乙型肝炎患者中均存在。 2 5例慢性无症状HBV感染者有 2例为HBV前C区突变株感染 ,突变发生率为 8%。 2 4例慢性乙型肝炎患者有 4例为HBV前C区1896位G -A突变株感染 ,突变发生率为 16 7%。两者无显著性差异。结论 慢性无症状HBV感染者中多数病人肝组织存在慢性肝炎的病理损害 ,为慢性肝炎病人。其炎症活动度及纤维化分级均低于慢性肝炎病人 ;HBV前C区1896位G -A突变与肝炎病情轻重无关
Objective To study the occurrence of G-A mutation in 1896 precancerous HBV-infected patients with chronic asymptomatic HBV infection and the degree of inflammatory activity and fibrosis of liver tissue, and to explore its clinical significance. Methods Hepatic inflammation activity and fiber grade were studied by hematoxylin and eosin staining (HE), Gorden-Sweet method and collagen fiber (GV) staining. PCR-RFLP method was used to detect 1896 G -A mutation. Results Of the 25 cases of chronic asymptomatic HBV infection except 3 cases (12%) without pathological changes of chronic hepatitis, the other cases were pathological changes of chronic hepatitis, of which 3 cases had obvious fibrosis around the portal area, fiber And 24 cases of chronic hepatitis B patients with clinical and pathological control study found that patients with chronic asymptomatic HBV infection in liver activity and fibrosis grade were lower than those with chronic hepatitis, and with the increased clinical manifestations of patients , Patients with inflammatory activity and liver fibrosis classification also increased. The 1896 G-A mutation in pre-HBV C region exists in both chronic asymptomatic HBV carriers and chronic hepatitis B patients. Two of the 25 cases with chronic asymptomatic HBV infection were infected with the pre-HBV C mutants, with a mutation rate of 8%. Four of 24 chronic hepatitis B patients were infected with 1896 G-A mutant in pre-HBV region, with a mutation rate of 16.7%. There was no significant difference between the two. Conclusion Most patients with chronic asymptomatic HBV infection have pathological damage of chronic hepatitis in the liver tissue, which is a chronic hepatitis patient. The inflammation activity and fibrosis grade were lower than those of patients with chronic hepatitis; Pre-HBV C-1896 G-A mutation was not associated with the severity of hepatitis