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目的探讨维吾尔族妇女宫颈上皮内瘤变(CIN)Ⅰ～Ⅲ级和子宫颈癌组织中miRNA-101的表达情况,并分析子宫颈癌中miRNA-101与人乳头瘤病毒(HPV)感染的关系。方法采用锁定核苷酸原位杂交技术(LNA-ISH)检测维吾尔族妇女子宫颈鳞状细胞癌、CINⅠ～Ⅲ级和慢性宫颈炎组织中miRNA-101的表达情况,并对子宫颈鳞状细胞癌中miRNA-101与HPV感染情况进行相关性分析。结果 miRNA-101在慢性宫颈炎,CINⅠ、Ⅱ、Ⅲ及子宫颈癌鳞状细胞中的表达率分别为80.00%、73.33%、46.67%、26.67%及10.00%(χ2=36.295,P=0.000)。子宫颈鳞状细胞癌中miRNA-101与HPV结果呈负相关(R=-0.767,P=0.000)。结论 miRNA-101参与子宫颈鳞状细胞癌的发生、发展,并且与HPV感染有关,miRNA-101可作为子宫颈癌筛查及早期诊断的分子指标之一。
Objective To investigate the expression of miRNA-101 in cervical intraepithelial neoplasia (CIN) Ⅰ ~ Ⅲ and cervical cancer in Uighur women and to analyze the relationship between miRNA-101 and HPV infection in cervical cancer. Methods The expression of miRNA-101 in cervical squamous cell carcinoma, CINⅠ-Ⅲlevel and chronic cervicitis in Uigur women was detected by LNA-ISH. The expression of miRNA-101 in cervical squamous cell carcinoma The correlation between miRNA-101 and HPV infection in cancer was analyzed. Results The expression rates of miRNA-101 in chronic cervicitis, CINⅠ, Ⅱ, Ⅲ and cervical squamous cell carcinoma were 80.00%, 73.33%, 46.67%, 26.67% and 10.00%, respectively . There was a negative correlation between miRNA-101 and HPV in cervical squamous cell carcinoma (R = -0.767, P = 0.000). Conclusions miRNA-101 is involved in the occurrence and development of cervical squamous cell carcinoma and is associated with HPV infection. MiRNA-101 can be used as a molecular marker for screening and early diagnosis of cervical cancer.