论文部分内容阅读
目的:建立测定枣仁安神颗粒中酸枣仁皂苷A含量的方法。方法:采用高效液相色谱法,应用Kromasil C18色谱柱(250mm×4.6mm,5μm);以乙腈-水(35∶65)为流动相;流速为1.0ml/min;柱温为30℃;气流:2.5ml/min;漂移管温度为110℃。结果:酸枣仁皂苷A线性范围为6～90μg/ml;平均回收率为96.2%,RSD为1.3%。结论:本试验方法简便、快捷,结果准确、重复性好,可用于该制剂中酸枣仁皂苷A的含量测定。
Objective: To establish a method for determination of Jujuboside A content in Zaoren Anshen granules. Methods: Kromasil C18 column (250 mm × 4.6 mm, 5 μm) was used in this study. The mobile phase consisted of acetonitrile and water (35:65), the flow rate was 1.0 ml / min, the column temperature was 30 ℃, : 2.5 ml / min; drift tube temperature 110 ° C. Results: Jujuboside A linear range of 6 ~ 90μg / ml; average recovery was 96.2%, RSD was 1.3%. Conclusion: The test method is simple, fast, accurate, reproducible, and can be used for the determination of Jujuboside A in this preparation.