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目的:考察大蒜提取物蒜氨酸在家兔肠道内的吸收特性,为新药研究提供实验数据。方法:采用家兔,利用外翻肠囊模型与光纤传感过程分析系统结合,实时原位测定蒜氨酸在家兔肠道内的浓度变化。研究蒜氨酸在家兔十二指肠、空肠、回肠、结肠的4个肠段,高、中、低3个浓度的吸收,每个肠段的每个浓度进行6样本(6只家兔,共计18只家兔)分析,每个肠段接触蒜氨酸的浓度根据半数致死量确定,配制蒜氨酸溶液的质量浓度分别为(700,50,30 mg·L-1),于光纤传感过程分析仪上进行检测。结果:家兔不同肠段对蒜氨酸的吸收能力大小:回肠>空肠>结肠>十二指肠;不同浓度下各肠段的吸收数An值空肠分别为(2.59±0.21,5.40±0.11,3.81±0.27);回肠分别为(5.25±0.07,3.02±0.19,4.09±0.18),各浓度An值均大于1.15,十二指肠分别为(0,0.95±0.16,1.09±0.28);结肠分别为(0.50±0.13,0.90±0.22,1.08±0.24),各浓度An值均<1.15。各浓度在4个肠段的Ke值除十二指肠段外(0,0.007±0.02,0.004±0.09)其余肠段的Ke值都<0.01 cm·min-1。结论:利用光纤传感系统实时测定蒜氨酸原料药在肠道的浓度变化,从而研究其在各肠段的吸收,结果表明蒜氨酸在除十二指肠段外其余肠段的渗透性很好。
Objective: To investigate the absorption characteristics of alliin from garlic extracts in the intestine of rabbits and provide experimental data for the study of new drugs. METHODS: Rabbits were used to determine the concentration change of alliin in the intestine of rabbits in real-time using real-time in situ quantification using an inverted luminal sac model and an optical fiber sensing process analysis system. To study the absorption of alliin in the duodenum, jejunum, ileum, and colon of the intestine, three concentrations of high, medium, and low, and 6 samples per concentration of each intestine (6 rabbits) A total of 18 rabbits were analyzed. The concentration of contact with alliin in each intestine was determined based on the median lethal dose, and the mass concentration of the formulated alliin solution was (700, 50, 30 mg·L-1), respectively. Sensing process analyzer for testing. RESULTS: The absorption capacity of alliin in different intestinal segments of rabbits was as follows: ileum> jejunum> colon> duodenum; the anesthetic value of intestinal anastomosis at different concentrations was jejunum (2.59±0.21, 5.40±0.11, respectively). 3.81 ± 0.27); ileum were (5.25 ± 0.07, 3.02 ± 0.19, 4.09 ± 0.18), An value of each concentration was greater than 1.15, duodenum was (0, 0.95 ± 0.16, 1.09 ± 0.28); For (0.50 ± 0.13, 0.90 ± 0.22, 1.08 ± 0.24), the An values for each concentration were <1.15. The Ke values of the four intestinal segments except for the duodenal segments (0, 0.007±0.02, 0.004±0.09) at each concentration were all <0.01 cm·min-1. Conclusion: The optical fiber sensing system was used to determine the concentration of alliin in the intestinal tract in real time, and its absorption in each intestine was studied. The results showed that the penetration of alliin in the other intestine except the duodenum segment. well.