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利用分子杂交原理，我们采用斑点杂交和原位杂交实验，证明了在正常大鼠肾脏有白细胞介素－１Ｉ型受体特异ｍＲＮＡ表达，分布于肾脏皮质和髓质。用兔抗大鼠肾皮质血清诱导产生的大鼠肾炎模型肾组织，其白细胞介素－１Ｉ型受体特异ｍＲＮＡ表达量明显高于正常肾组织，尤其髓质部分较为显著。肾脏固有细胞如上皮细胞及肾小球中某些细胞（可能为系膜细胞）在正常情况下有少量介素－１Ｉ型受体ｍＲＮＡ表达，随着炎症的产生表达量明显增高。我们的实验结果为白细胞介素－１在肾脏中的功能通过结合白细胞介素－１特异受体的作用机制，白细胞介素－１在肾脏中的自泌与旁泌作用方式，在分子水平上提供了实验依据。
Using the principle of molecular hybridization, we used dot blot hybridization and in situ hybridization experiments to demonstrate that interleukin-1 receptor type I-specific mRNA was expressed in the kidney of normal rats and distributed in the renal cortex and medulla. Rat kidney nephritis model induced by rabbit anti-rat renal cortex serum expression of interleukin-1 receptor-specific mRNA was significantly higher than normal kidney tissue, especially in the medullary part of the more significant. Some innate cells in the kidney such as epithelial cells and some cells in the glomerular (which may be mesangial cells) under normal circumstances have a small amount of mRNA expression of type I receptor, with the expression of inflammation was significantly increased. Our experimental results show that interleukin-1 (IL-1) functions in the kidney by binding to the specific mechanism of interleukin-1 receptor, interleukin-1 in the kidney in an autocrine and paracrine manner, at the molecular level Provided experimental basis.