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目的：研究短程大剂量粒细胞集落刺激因子（Ｇ－ＣＳＦ）对外周血造血干细胞的动员作用。方法：采用短程大剂量Ｇ－ＣＳＦ对１１例患者进行外周血造血干细胞动员，Ｇ－ＣＳＦ５μｇ／ｋｇ皮下注射，每日２次，共３天，动员当天及第４天（单采日），分别取骨髓及外周血进行常规检查，ＣＦＵ－ＧＭ、ＢＦＵ－Ｅ、ＣＦＵ－ｍｉｘ集落培养及ＣＤ３４、ＣＤ３８细胞检测。结果：动员后患者骨髓增生明显活跃，外周血白细胞计数明显升高。骨髓、外周血ＣＦＵ－ＧＭ、ＢＦＵ－Ｅ及ＣＦＵ－ｍｉｘ集落，ＣＤ３４＋ＣＤ３８＋细胞、ＣＤ３４＋ＣＤ３８－细胞均较动员之前明显增多（Ｐ＜０．０５）。一次单采的单个核细胞（ＭＮＣ）平均为１．５６×１０８／ｋｇ。冷冻后ＭＮＣ、ＣＦＵ－ＧＭ、ＢＦＵ－Ｅ及ＣＤ３４＋ＣＤ３８＋细胞的恢复率为８０％（Ｐ＞０．０５）；ＣＦＵ－ｍｉｘ的恢复率为６０％，但ＣＤ３４＋ＣＤ３８－细胞冷冻后减少（Ｐ＜０．０５）。回输患者体内ＭＮＣ的平均集落数分别为：ＣＦＵ－ＧＭ６．７０×１０４／ｋｇ；ＢＦＵ－Ｅ１．６０×１０４／ｋｇ；ＣＦＵ－ｍｉｘ０．３２×１０４／ｋｇ；ＣＤ３４＋ＣＤ３８＋细胞１．６０×１０６／ｋｇ；ＣＤ３４＋ＣＤ３８＋细胞０．２３×１０６?
AIM: To investigate the mobilization of peripheral blood hematopoietic stem cells by short-term high-dose granulocyte-colony stimulating factor (G-CSF). Methods: 11 cases of peripheral blood hematopoietic stem cell mobilization and 5-g / kg G-CSF subcutaneously were injected subcutaneously twice a day for 3 days with short-range and high-dose G-CSF. The mobilization day and the fourth day Bone marrow and peripheral blood were taken for routine examination, CFU-GM, BFU-E, CFU-mix colonies and CD34, CD38 cells. Results: Myeloid hyperplasia was significantly active after mobilization, and the peripheral blood leukocyte count was significantly increased. CFU-GM, BFU-E and CFU-mix colonies, CD34 + CD38 + cells and CD34 + CD38- cells in bone marrow and peripheral blood were significantly increased compared with before mobilization (P <0.05). A single apheresis mononuclear cells (MNC) averaging 1.56 × 108 / kg. The recovery rate of MNC, CFU-GM, BFU-E and CD34 + CD38 + cells after cryopreservation was 80% (P> 0.05). The recovery rate of CFU-mix was 60%, but the number of CD34 + CD38- cells decreased after freezing (P < 05). The average colony numbers of MNC in the transfused patients were CFU-GM6.70 × 104 / kg, BFU-E1.60 × 104 / kg, CFU-mix0.32 × 104 / kg and CD34 + CD38 + cells 1.60 × 106 / kg; CD34 + CD38 + cells 0.23 × 106?