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目的：探索优化的原代鸭肝细胞分离方法，为鸭肝细胞在体外研究中的应用奠定基础。方法：分别采用胶原酶（Ⅰ型与Ⅳ型）经门静脉或胆总管原位灌流分离鸭肝细胞，观测肝细胞产量、存活率及其形态。结果：１．经门静脉原位灌流分离鸭肝细胞效果明显优于经胆总管灌流法；２．以右心室为灌流液流出道分离鸭肝细胞效果明显优于经下腔静脉流出道分离法：３．Ⅰ型胶原魂与Ⅳ型胶原酶灌流分离鸭肝细胞效果相当。结论：采用Ⅰ型或Ⅳ型胶原酶经门静脉－右心室原位灌流分离鸭肝细胞为一较好的方法，有利于鸭肝细胞为模型的体外研究工作的开展。
Objective: To explore the optimized method of primary duck hepatocyte isolation and lay a foundation for the application of duck hepatocyte in vitro. Methods: Duck liver cells were isolated by in situ perfusion with collagenase (type Ⅰ and type Ⅳ) via portal vein or common bile duct. The production, survival rate and morphology of hepatocytes were observed. Results: 1. In situ perfusion through the portal vein of duck hepatocytes was significantly better than that of the common bile duct perfusion method; 2. The right ventricle as perfusion fluid outflow tract isolation duck hepatocytes significantly better than the inferior vena cava outflow tract separation method: 3. Collagen type Ⅰ and type Ⅳ collagenase perfused duck liver cells perfusion effect. Conclusion: It is a good method to isolate duck hepatocytes by in situ perfusion of type Ⅰ or type Ⅳ collagenase via portal vein - right ventricle, which is in favor of the in vitro study of duck hepatocytes.