目的建立一种人脑膜瘤细胞原代培养简单有效的方法。方法新鲜脑膜瘤组织取自福建协和医院神经外科25例连续治疗的脑膜瘤手术病人,以胰蛋白酶消化法分离细胞,用含10%胎牛血清的DMEM培养液进行培养并传代,显微镜下观察脑膜瘤细胞生长状况。上皮膜抗原(EMA)、波形蛋白(Vimentin)对脑膜瘤细胞进行免疫组化鉴定。结果用胰酶消化法培养脑膜瘤细胞,方法简单,细胞形态典型;EMA、Vimentin表达阳性,鉴定为脑膜瘤细胞。结论单一的胰蛋白酶消化法是一种简单有效的脑膜瘤细胞原代培养的方法。 Objective To establish a simple and effective method for primary culture of human meningioma cells. Methods The fresh meningiomas were obtained from 25 consecutive patients with meningiomas undergoing surgery at Department of Neurosurgery, Union Hospital, Fujian Province. Cells were isolated by trypsin digestion and cultured in DMEM containing 10% fetal bovine serum. The cells were observed under a microscope Tumor cell growth status. Epithelium membrane antigen (EMA), vimentin (Vimentin) on the meningioma cells immunohistochemical identification. Results Incubation of meningioma cells with trypsin digestion method was simple, cell morphology was typical; EMA, Vimentin expression was positive, identified as meningioma cells. Conclusion A single trypsin digestion method is a simple and effective method of primary culture of meningioma cells.