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目的 :观察糖尿病肾病大鼠血糖、胆固醇、甘油三脂含量的变化以及消渴颗粒剂对其的影响。方法 :采用 3/ 4肾切除 ,腹腔一次性注射STZ复制大鼠糖尿病肾病模型。将动物分为模型组、消渴颗粒剂治疗组、阳性药对照组和假手术组。每组大鼠于注射STZ后 1、2、3、4、5周尾尖取血测定空腹血糖、血清肌酐、血清胆固醇和血清甘油三脂含量。各组大鼠于注射STZ后 6周杀检 ,进行肾脏形态学观察。结果 :注射STZ后 6周 ,模型组大鼠有不同程度的肾小球硬化。中药治疗组上述病变明显轻于病理组。注射STZ后第 1、2、3、4、5周 ,模型组血清肌酐明显高于假手术组 (P <0 0 5 ) ,治疗组则明显低于模型组和阳性对照组。注射STZ后 1、2、3、4、5周模型组空腹血糖、血清胆固醇明显高于假手术组 (P <0 0 5 ) ,血清甘油三脂在注射STZ后 1、2、3、5周明显高于假手术组 (P <0 0 5 ) ;消渴颗粒剂治疗组和阳性对照药组与模型组同期比较病变明显减轻。空腹血糖和血清胆固醇、甘油三脂含量呈正相关。结论 :采用此方法成功地复制了大鼠糖尿病肾病模型 ,糖尿病肾病大鼠血糖增高可能引起血脂增高 ,消渴颗粒剂治疗糖尿病肾病的途径之一是降低大鼠血糖、血清胆固醇、血清甘油三脂含量 ,从而改善肾小球病变
Objective: To observe the change of blood glucose, cholesterol and triglyceride content in rats with diabetic nephropathy and the effect of Xiaoke granules on it. METHODS: A 3/4 nephrectomy was used to induce rat model of diabetic nephropathy by intraperitoneal injection of STZ. Animals were divided into model group, Xiaoke granule treatment group, positive drug control group and sham operation group. Fasting blood glucose, serum creatinine, serum cholesterol, and serum triglyceride levels were measured at 1, 2, 3, 4 and 5 weeks after injection of STZ in each group of rats. Rats in each group were killed 6 weeks after STZ injection for renal morphology observation. RESULTS: Six weeks after the injection of STZ, rats in the model group had different degrees of glomerulosclerosis. The Chinese medicine treatment group was significantly lighter than the pathological group. At weeks 1, 2, 3, 4 and 5 after STZ injection, serum creatinine in the model group was significantly higher than that in the sham group (P < 0.05). The treatment group was significantly lower than the model group and the positive control group. The fasting blood glucose and serum cholesterol in the model group were significantly higher than those in the sham operation group at 1, 2, 3, 4 and 5 weeks after injection of STZ (P < 0.05). Serum triglyceride levels were 1, 2, 3, and 5 weeks after STZ injection. Significantly higher than the sham operation group (P < 0.05); Diabetes granules treatment group and positive control drug group and the model group compared to the same period significantly reduced lesions. Fasting blood glucose was positively correlated with serum cholesterol and triglyceride levels. Conclusion: The rat model of diabetic nephropathy was successfully replicated by this method. The increase of blood glucose in diabetic nephropathy rats may cause blood lipids to increase. One of the ways that Xiaoke granules treat diabetic nephropathy is to reduce blood glucose, serum cholesterol and serum triglyceride in rats. Content, thereby improving glomerular disease