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目的:通过对犬脑断层解剖、颅脑磁共振三维扫描以及脑积水模型建立的研究,探讨犬脑积水动物模型建立的方法。方法:选择正常杂种雄性犬8只,体重15kg左右,行颅脑磁共振三维扫描。其中3只行颅脑断层解剖切片;5只测量各个脑室多个径线,手术暴露出環枕硬脑膜,将导管的尖端轻柔推进到第四脑室内的头侧半,缓慢注入氰基丙烯酸酯凝胶0.3ml,使之充满第四脑室。实验数据经t检验。结果:通过对正常犬及犬脑积水模型脑室系统的断层解剖及颅脑磁共振三维扫描,获得了犬脑室系统的一系列数据,为建立犬脑积水动物模型及脑积水程度的判定提供了数据资料。诱导后5条犬,其中1条为重度脑室扩大(Evan’s比率改变的百分数为168%),2条中度扩大(57%、75%),2条为轻度扩大(15%、25%)。结论:①犬脑积水模型相对于其它大型哺乳动物模型,价格低,易饲养,模型建立成功率高,是比较适合用于脑积水诊断及治疗方面研究的动物模型之一;②研究中发现犬三脑室内的中间块较大,即使较重的脑积水,现有的神经内窥镜达到三脑室底较困难,因此不是进行神经内窥镜第三脑室造瘘术研究的理想动物模型。
OBJECTIVE: To investigate the establishment of animal models of canine hydrocephalus by studying the anatomy of canine brain, three-dimensional magnetic resonance imaging of brain and establishment of hydrocephalus model. Methods: Eight normal male hybrid dogs were selected and their body weight was about 15kg. Three-dimensional magnetic resonance MR scanning was performed. Of which 3 were anatomical sections of craniocerebral tomography; 5 were measured a number of radial lines of each ventricle, the operation exposed the ring dura mater, the tip of the catheter gently pushed to the head of the fourth ventricle half of the slow intravenous injection of cyanoacrylate Gel 0.3ml, so full of the fourth ventricle. Experimental data by t test. Results: A series of data on canine ventricular system were obtained through tomography and three-dimensional brain magnetic resonance imaging of normal dogs and dogs with hydrocephalus model, which was used to determine the animal model of hydrocephalus and hydrocephalus Provided data Five dogs were induced, one of whom had severe ventricular enlargement (168% of Evan’s ratio change), two moderately enlarged (57%, 75%) and two mildly enlarged (15%, 25% . Conclusions: (1) Compared with other large mammalian models, the model of hydrocephalus in dogs is low in price, easy to raise and has a high success rate in establishing models. It is one of the animal models that is more suitable for the diagnosis and treatment of hydrocephalus. It was found that the middle block of the dog’s third ventricle was large. Even with severe hydrocephalus, the existing neuroendoscopy was more difficult to reach the third ventricle, and was therefore not the ideal animal to perform the third ventricle ostomy in neuroendoscopy model.