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目的:通过离体实验,观察机械生长因子(MGF)对于骨骼肌卫星细胞(SC)的激活作用及促进其增殖的量效关系,探讨MGF对SC在细胞水平的调节机制,为骨骼肌损伤的修复提供理论依据。方法:原代SC取自4周龄雄性SD大鼠双侧的腓肠肌与比目鱼肌。使用不同浓度的MGF干预第三代细胞后测定增殖效果。血清饥饿后通过MGF和DMEM干预骨骼肌卫星细胞,使用流式细胞仪检测其所处细胞周期,判断激活情况。结果:干预48h后,25ng/ml组、50ng/ml组A值显著高于对照组(P<0.01),100ng/ml组、200ng/ml组与对照组之间差异没有显著性(P>0.05);96h后25ng/ml组、50ng/ml组、100ng/ml组、200ng/ml组之间差异没有显著性(P>0.05)。25ng/mlMGF干预G0期的SC24h后MGF组低于对照组(P<0.01)。结论:MGF可以促进SC增殖;MGF可以激活4周龄SD大鼠的G期SC。
OBJECTIVE: To investigate the effect of MGF on the activation and proliferation of skeletal muscle satellite cells (SCs) in vitro and to explore the regulatory mechanism of MGF on SC at the cellular level, Repair provides a theoretical basis. Methods: Primary SC was taken from bilateral gastrocnemius and soleus muscle of 4-week-old male Sprague-Dawley rats. Proliferation effect was measured after intervention of the third generation of cells with different concentrations of MGF. After serum starvation, the skeletal muscle satellite cells were intervened by MGF and DMEM. The cell cycle of the skeletal muscle cells was detected by flow cytometry to determine the activation status. Results: After 48 hours of intervention, the A value in 25ng / ml group and 50ng / ml group was significantly higher than that in control group (P <0.01). There was no significant difference between 100ng / ml group and 200ng / ml group and control group ). There was no significant difference between 25ng / ml group, 50ng / ml group, 100ng / ml group and 200ng / ml group after 96h (P> 0.05). MGF group at 25ng / ml MGF intervention was lower than that of control group at G0 stage (P <0.01). Conclusion: MGF can promote SC proliferation. MGF can activate G stage SC in 4-week-old SD rats.