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目的:探讨系统性红斑狼疮(SLE)患者外周血单个核细胞中核因子(NF)-κB信号通路中IκB激酶(IκK)-α、IκKβ、IκB-α mRNA的定量表达水平,及与SLE病情活动程度的相关性。方法:采集110例SLE患者和50名正常人外周血,抽提总RNA并反转录成cDNA,运用实时定量PCR法在基因测序仪上检测IκK-α、IκK-β、IκB-α mRNA的表达水平。结果:①SLE患者组的IκK-α、IκK-β mRNA定量表达水平显著高于正常对照组(P<0.01);未使用糖皮质激素的初发SLE患者IκK-α、IκK-β mRNA定量表达水平显著高于已使用糖皮质激素的SLE患者(P<0.05);②SLE患者组的IκB-α mRNA定量表达水平显著低于正常对照组(P<0.01);未使用糖皮质激素的初发SLE患者IκB-αmRNA定量表达水平显著低于已使用糖皮质激素的SLE患者(P<0.01);③IκB-α mRNA的表达与IκK-α、IκK-β mRNA表达水平呈负相关(P=0.000);④IκB-α mRNA的表达与SLE疾病活动指数(SLEDAI)积分呈负相关(P<0.01)。结论:SLE患者体内NF-κB信号通路处于高活性状态,且与疾病活动程度相关,阻抑NF-κB过度活化可能有利于SLE的治疗。
Objective: To investigate the quantitative expression of IκB kinase (IκK) -α, Iκκβ, IκB-α mRNA in nuclear factor (NF) -κB signaling in peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE) Degree of relevance. Methods: Peripheral blood was collected from 110 patients with SLE and 50 normal controls. Total RNA was extracted and reverse transcribed into cDNA. Real-time PCR was used to detect IκK-α, IκK-β and IκB-α mRNA The expression level. Results: ①Quantitative expression of IκK-αand IκK-βmRNA in SLE patients was significantly higher than that in the normal control group (P <0.01); Quantitative expression of IκK-αand IκK-βmRNA in primary SLE patients without glucocorticoid (P <0.05). ② The quantitative expression level of IκB-α mRNA in patients with SLE was significantly lower than that in the control group (P <0.01); Patients with SLE without glucocorticoid The expression level of IκB-α mRNA was significantly lower than that of patients with SLE (P <0.01). ③ The expression of IκB-α mRNA was negatively correlated with the expression of IκK-α and IκK-β mRNA (P = 0.000) The expression of -α mRNA was negatively correlated with the SLE disease activity index (SLEDAI) score (P <0.01). CONCLUSIONS: The NF-κB signaling pathway is highly active in SLE patients and is related to the degree of disease activity. Suppression of NF-κB overactivation may be beneficial to the treatment of SLE.