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目的:探讨凋亡抑制蛋白Survivin、bcl-2蛋白在非小细胞肺癌(NSCLC)组织中的表达与不同临床病理特征的关系及二者在肺癌发生过程中可能存在的相关性。方法:采用免疫组化法的链霉菌抗生物素过氧化酶(S-P)法检测80例NSCLC组织和20例正常肺组织中Survivin、bcl-2蛋白表达情况。结果:非小细胞肺癌组织中Survivin、bcl-2蛋白阳性表达率分别为61.3%、50%,而在肺良性病变组织中阳性表达率分别为0%、10%,两者在两组中的表达均有显著性差异(P<0.05);Survivin蛋白在～期肺癌组织中阳性表达率72.0%显著高于I～II期肺癌组织的43.3%(P<0.05),bcl-2蛋白在鳞癌组的阳性表达率62.2%高于腺癌组的34.3%(P<0.05);Survivin、bcl-2蛋白在肺癌组织中的表达呈正相关关系。结论:Survivin蛋白表达与肺癌的TNM分期密切相关,Survivin、bcl-2可以作为判断病情和评价预后的指标。
OBJECTIVE: To investigate the relationship between the expression of Survivin and bcl-2 protein in non-small cell lung cancer (NSCLC) and their clinicopathological features and the possible relationship between them in lung carcinogenesis. Methods: The expression of Survivin and bcl-2 protein in 80 cases of NSCLC and 20 cases of normal lung tissues was detected by immunohistochemical method of Streptomyces anti-biotin peroxidase (S-P). Results: The positive rates of Survivin and bcl-2 protein expression in non-small cell lung cancer tissues were 61.3% and 50%, respectively. The positive rates of Survivin and bcl-2 protein in benign lung lesions were 0% and 10%, respectively (P <0.05). The positive rate of Survivin protein in stage-Ⅲ lung cancer was 72.0%, which was significantly higher than that in stage I-II lung cancer (43.3%, P <0.05). The expression of bcl- The positive expression rate of Survivin and bcl-2 in lung cancer tissues was 62.2% higher than that in adenocarcinoma tissues (34.3%, P <0.05). Conclusion: Survivin protein expression is closely related to TNM stage of lung cancer. Survivin and bcl-2 may be used as indicators to judge the prognosis of the patients.