一点红对STZ诱导糖尿病大鼠的降血糖作用及机制研究

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目的:研究一点红提取物对链脲佐菌素(STZ)诱导糖尿病大鼠模型的降糖作用及其机制。方法:选取6~7周龄SPF级雄性SD大鼠72只,实验分成6组,每组12只,除正常对照组外,糖尿病成模大鼠随机分为5组,分别为模型组、盐酸二甲双胍(150 mg/kg)阳性对照组及一点红提取物低(1 g/kg)、中(5 g/kg)、高(10 g/kg)剂量组,连续灌胃给药28 d。结果:与正常对照组比较,模型组大鼠血清TG、TC、LDL-C、FBG、MDA显著升高(P<0.05),HDL-C、Fins、C-P、SOD显著降低(P<0.05);与模型组比较,各给药组血清TG、TC、LDL-C、FBG、MDA显著降低(P<0.05),HDL-C、Fins、C-P、SOD显著升高(P<0.05);组织病理学检查显示模型组胰岛数目减少、细胞排列紊乱、胞浆减少、胰岛内细胞边界不清、不规则。一点红各组大鼠随着药物剂量升高,组织病理学改变逐渐改善。结论:一点红提取物对STZ诱导糖尿病大鼠具有一定的降糖作用,并能提高模型动物血清SOD水平,降低血清MDA及血脂水平,对胰岛细胞具有一定的保护作用。 Objective: To study the hypoglycemic effect and mechanism of little red extract on streptozotocin (STZ) -induced diabetic rat model. Methods: Seventy-two male Sprague-Dawley (SD) rats aged 6-7 weeks were divided into 6 groups (n = 12). Except normal control group, diabetic rats were randomly divided into 5 groups: model group, The rats in the control group and metoprogin (150 mg / kg) group were given low dose (1 g / kg), medium dose (5 g / kg) and high dose (10 g / kg) Results: Compared with the normal control group, the levels of serum TG, TC, LDL - C, FBG and MDA in model group were significantly increased (P <0.05) and HDL - C, Fins, CP and SOD were significantly decreased (P < Compared with the model group, the levels of TG, TC, LDL-C, FBG and MDA were significantly decreased (P <0.05), HDL-C, Fins, CP and SOD were significantly increased Examination showed that the model group, the number of islets decreased, disordered cells, reduced cytoplasm, intracellular islet cell boundaries unclear and irregular. Little red rats in each group with the drug dose increased, histopathological changes gradually improved. Conclusion: The red extract of STZ-induced diabetic rats have a certain hypoglycemic effect, and can improve the model animal serum SOD levels, lower serum MDA and lipid levels, has a certain degree of protection of pancreatic islet cells.
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