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目的探讨人食管下括约肌的套索纤维和钩状纤维对CCK-8和胃泌素-17的反应特点,以及胆囊收缩素受体对两者的调节作用。方法运用离体组织张力测量技术,得出套素纤维和钩状纤维对CCK-8和胃泌素-17的最大收缩效应值(Emax),以及特异性胆囊收缩素受体拮抗剂对 CCK-8的pKB值。结果套索纤维对CCK-8和胃泌素-17产生了较强的浓度依赖性收缩反应 [Emax值:(5．18±0．50)mN／mm2和(4．91±0．95)mN／mm2],而钩状纤维的收缩强度[Emax值: (0．73±0．23)mN／mm2和(0．72±0．14)mN／mm2]显著低于套索纤维(P<0．001;P<0．01)。 CR1409和CR2945对CCK-8引发的套索纤维收缩均产生了浓度依赖性抑制作用,前者的pKB值 (7．51±0．11)大于后者(7．15±0．11)(P<0．05)。结论套索纤维对CCK-8和胃泌素-17刺激的收缩强度显著大于钩状纤维,CCKA受体在其中发挥着更为重要的作用。
Objective To investigate the response of laryngeal fibers and hooked fibers of human lower esophageal sphincter to CCK-8 and gastrin-17 and the regulation of cholecystokinin receptor on them. Methods Tissue tension measurement technique was used to determine the maximal systolic effect (Emax) of CCK-8 and HGF on CCK-8 and hook-fibrin fibers and the effect of specific cholecystokinin receptor antagonist on CCK- 8 pKB value. Results Lasso fibers produced strong concentration-dependent contractile responses to CCK-8 and gastrin-17 [Emax values: (5.18 ± 0.50) mN / mm2 and (4.91 ± 0.95) (0.73 ± 0.23) mN / mm2 and (0.72 ± 0.14) mN / mm2] were significantly lower than those of lasso fibers (P <0.001; P <0.01). CR1409 and CR2945 produced a concentration-dependent inhibition of the contractility of lasso fibers induced by CCK-8, the former had a higher pKB value (7.51 ± 0.11) than the latter (7.15 ± 0.11) (P < 0.05). CONCLUSION: The sling fibers induced greater contractile effects on CCK-8 and gastrin-17 than hook-shaped fibers, and CCKA receptors play a more important role.