目的探讨体内多种组织细胞对DNA氧化损伤效应的敏感性及损伤后的自身修复能力。方法以H2O2 作为氧化损伤剂 ,对离体的小鼠肝、肾、脾细胞进行染毒 ,用彗星试验观察各种细胞的DNA氧化损伤效应与修复动力学改变。结果H2O2 能诱导三种细胞DNA氧化损伤 ,其损伤的敏感性依次为 :脾细胞>肾细胞>肝细胞 ;修复试验显示肝细胞修复能力最强 ,修复时间最短 ,脾细胞次之 ,而肾细胞在2h内几乎无修复。结论体内组织细胞对氧化损伤的敏感性及修复能力差异很大 ,彗星试验在一定程度上可以检测外源化学物的DNA氧化损伤效应 Objective To investigate the sensitivity of various tissue cells to DNA oxidative damage and their self-repair ability after injury. Methods H2O2 was used as an oxidant-damaging agent to infect isolated mouse liver, kidney and spleen cells. The effects of oxidative DNA damage and repair kinetics of various cells were observed by comet assay. Results H2O2 induced oxidative DNA damage in three kinds of cells. The sensitivity of the injury was spleen cells> kidney cells> hepatocytes. The repair test showed that the repair ability of hepatocytes was the strongest, the repair time was the shortest, followed by the spleen cells, Almost no repair within 2h. Conclusion The sensitivity of tissue cells to oxidative damage and their ability to repair vary widely. The comet assay can detect DNA oxidative damage effects of foreign chemicals to some extent