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本文采用钙荧光探剂 Ｆｌｕｏ—３／ＡＭ染色法，定量研究了双歧杆菌１０２７株、肠致病性大肠杆菌（ＥＰＥＣ）对体外肠上皮细胞Ｌｏｖｏ细胞株粘附的钙信号传递机制。结果表明，双歧杆菌１０２７株粘附可引起Ｌｏｖｏ细胞内Ｃａ~＋２随时间延长而梯度升高，但双歧杆菌１０２７株的作用远不如ＥＰＥＣ明显。同时发现双歧杆菌粘附引起Ｌｏｖｏ。细胞内Ｃａ~２＋升高主要源于细胞外Ｃａ~２＋内流所致，这与ＥＰＥＣ粘附引起宿主细胞内Ｃａ~２＋升高主要源于细胞内Ｃａ~２＋储池的Ｃａ~２＋释放不同。ＥＰＥＣ粘附引起宿主细胞内Ｃａ~２＋大幅度升高是其致病的重要信号传递基础；而双歧杆菌粘附仅引起宿主细胞内Ｃａ~２＋轻度升高，可能是其作为生理性细菌与肠上皮细胞和谐共生的信号传递基础。
In this study, calcium signaling Fluo-3 / AM staining was used to quantitatively study the calcium signaling pathway of 1027 strains of Bifidobacterium and of enteropathogenic E.coli (EPEC) on Lovo cells in vitro. The results showed that the adhesion of Bifidobacterium 1027 could cause the Ca 2 + in Lovo cells to increase with time, but the effect of Bifidobacterium 1027 was far less than that of EPEC. Also found that Bifidobacterium adhesion caused Lovo. The increase of intracellular Ca ~ (2 +) was mainly due to the influx of extracellular Ca ~ (2+), which was different from the Ca ~ (2 +) release in intracellular Ca ~ . EPEC adhesion caused by a large increase in Ca ~ (2 +) in host cells is an important signal pathogenic basis; while Bifidobacterium adhesion only caused a slight increase in Ca ~ 2 + in host cells, The basis of signaling symbiosis with intestinal epithelial cells.