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本文以黄瓜为实验材料,研究了不同温度下植物叶片交替氧化酶呼吸途径(AOX途径)对光破坏防御贡献的差异及其机理。结果表明,低温不仅抑制了AOX途径的活性,还抑制了“Malate-OAA”穿梭,导致光合作用产生的过剩还原力NAD(P)H的消耗减少,低温下抑制AOX途径后没有加重叶片光抑制;而高温下AOX途径活性的上调有效消耗了通过“Malate-OAA”穿梭机制转运而来的过剩还原力,缓解了光合电子传递链的过度还原,并且AOX途径受抑后,叶片光抑制显著增加。上述结果表明AOX途径在低温下对光破坏防御的贡献受到明显限制,而在高温下AOX途径的上调增加了其对光破坏防御的贡献。
In this paper, cucumber was used as experimental material to study the difference and mechanism of light-defensive defense contribution of alternate oxidase respiration pathway (AOX pathway) at different temperatures. The results showed that low temperature not only inhibited the activity of AOX pathway, but also inhibited the shuttle of “Malate-OAA ”, resulting in the decrease of NAD (P) H depletion caused by photosynthesis. Photoinhibition. However, up-regulation of AOX pathway activity at high temperature effectively depleted the excess reducing power transported by the “Malate-OAA” shuttle mechanism and alleviated the over-reduction of photosynthetic electron transport chains. After the inhibition of AOX pathway, Photoinhibition increased significantly. The above results indicate that the contribution of the AOX pathway to photodisruption defenses is significantly limited at low temperatures, while the upregulation of AOX pathway at elevated temperatures increases its contribution to photodrodcter defenses.