目的建立复方新斯的明眼用凝胶中维生素B6与有关物质的含量测定方法。方法采用高效液相色谱法,EclipseXDB-C18色谱柱(4.6 mm×150 mm,5μm)。流动相:醋酸三乙胺溶液。检测波长:290 nm。结果线性范围为1~50μg.mL-1,r=0.999 9(n=5)。低、中、高三个浓度回收率为(100.59±0.57)%,(100.97±0.38)%,(102.20±0.06)%。结论本方法简便快速、专属性好、重复性好、易操作。 Objective To establish a method for the determination of vitamin B6 and related substances in the compound neostigmine gel. Methods High performance liquid chromatography (HPLC) was used with EclipseXDB-C18 column (4.6 mm × 150 mm, 5 μm). Mobile phase: triethylamine acetate solution. Detection wavelength: 290 nm. Results The linear range was 1 ~ 50μg.mL-1, r = 0.999 9 (n = 5). The recoveries of low, medium and high concentrations were (100.59 ± 0.57)%, (100.97 ± 0.38)% and (102.20 ± 0.06)%, respectively. Conclusion The method is simple, rapid, specific, reproducible and easy to operate.