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目的对烟酸芽胞杆菌Bacillus niacini FJAT-14202来源的苯乳酸成分进行分析与检测。方法采用液相色谱/四级杆飞行时间质谱(liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry)分析烟酸芽胞杆菌FJAT-14202发酵液中胞外代谢物的成分。利用Mass Hunter软件,对原始数据进行分子特征提取,通过Metlin代谢物质谱数据库检索比对获得代谢物的信息。结果在烟酸芽胞杆菌FJAT-14202发酵液中检测到811种代谢物,通过Metlin谱库搜索获得初步鉴定的有165种。其中,发现了具有生物活性的物质苯乳酸,其匹配得分达到96.52,占发酵液总代谢物相对含量的5.31%,保留时间分别为2.1057 min,精确质量数为166.0552。结论苯乳酸的发现为烟酸芽胞杆菌的开发与利用提供理论依据。
OBJECTIVE To analyze and detect the components of the benzene lactic acid from Bacillus niacini FJAT-14202. Methods The constituents of extracellular metabolites in Bacillus thuringiensis FJAT-14202 fermentation broth were analyzed by liquid chromatography-quadrupole time-of-flight mass spectrometry. Mass Hunter software was used to extract the molecular features of the original data and obtain metabolites through Metlin Metabolite Mass Spectrometry Database. Results A total of 811 metabolites were detected in Bacillus nicotianae FJAT-14202 fermentation broth and 165 strains were initially identified by Metlin library search. Among them, the bio-active substance of benzene-lactic acid was found, with a match score of 96.52, accounting for 5.31% of the total metabolite content of the fermentation broth. The retention time was 2.1057 min, and the exact mass number was 166.0552. Conclusion The discovery of benzene lactic acid provides a theoretical basis for the development and utilization of Bacillus subtilis.