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为获得高而且稳定的小麦单倍体胚得胚率,从而使小麦×玉米远缘杂交产生小麦单倍体的方法应用于小麦育种实践,采用三种不同基因型冬小麦材料在四种不同生长环境条件下与玉米进行远缘杂交,使用含四种不同体积浓度二甲基亚砜(DMSO)的100 mg/L 2,4-D溶液进行激素处理,获得小麦单倍体胚。结果表明,三种冬小麦材料与玉米杂交都能获得单倍体胚,但得胚率存在差异,以小偃22最高(9.9%);不同生长环境条件的得胚率也存在差异,以温室条件下的最高(11.2%);不同体积浓度激素处理的得胚率无差异,平均为7.8%;各处理中以温室条件下陕农757用含3%DMSO的100 mg/L 2,4-D溶液进行激素处理的平均得胚率(22.6%)最高。
In order to obtain a high and stable blastocyst embryo rate in wheat so that the wheat × maize distant hybridization of wheat haploid method used in wheat breeding practice, using three different genotypes of winter wheat material in four different growth environments Were hybridized with maize by distant crosses. Hormones were treated with 100 mg / L 2,4-D solution containing four different concentrations of dimethyl sulfoxide (DMSO) to obtain wheat haploid embryos. The results showed that haploid embryos could be obtained from the cross of three kinds of winter wheat and maize, but the difference of embryo rate was found, the highest was Xiaoyan 22 (9.9%). There were also differences in germination rate under different growth conditions. (11.2%). There was no difference in the embryo rate of embryos treated with different concentrations of hormone, with an average of 7.8%. In each treatment, Shannong 757 was treated with 100 mg / L 2,4-D The average embryogenic rate (22.6%) of hormone solution treatment was the highest.