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目的:探讨银杏总黄酮对异丙基肾上腺素致心力衰竭大鼠的心功能和血管紧张素Ⅱ(AngⅡ)、血清内皮素1(ET-1)的影响。方法:选用SPF级雄性SD大鼠40只,随机分为4组:空白组、模型组、银杏总黄酮组和美托洛尔组。除空白组外均给予皮下注射300mg/kg异丙基肾上腺素2次,造模成功后银杏总黄酮组给予200mg·kg-1·d-1的银杏总黄酮提取物,美托洛尔组给予美托洛尔20mg/kg,其余灌胃给予相同体积的0.9%氯化钠溶液,连续8周。实验结束后测定大鼠心功能,血清内ET1和AngⅡ含量和病理积分,并取心肌观察病理形态学特征。结果:银杏总黄酮组和美托洛尔组左心室射血分数、室间隔厚度和左心室后壁厚度降低,左心室舒张末内径、左心室收缩末内径升高,与模型组有显著差异(P<0.05),表明治疗后心功能有所提高;银杏总黄酮组ET-1、AngⅡ含量和病理评分明显下降,与模型组相比差异有统计学意义(P<0.05)。结论:银杏总黄酮可改善心力衰竭大鼠的心功能,其作用机制可能与降低ET-1、AngⅡ的含量有关。
Objective: To investigate the effects of ginkgo flavonoids on cardiac function and the levels of serum angiotensin Ⅱ (Ang Ⅱ) and endothelin-1 (ET-1) in rats with heart failure caused by isoproterenol. Methods: Forty SPF male SD rats were randomly divided into 4 groups: blank group, model group, ginkgo flavonoids group and metoprolol group. Except the blank group were given subcutaneous injection of 300mg / kg isoproterenol twice, after the success of modeling ginkgo total flavonoids group was given 200mg · kg-1 · d-1 ginkgo total flavonoids extract, metoprolol group given Metoprolol 20mg / kg, the remaining gavage given the same volume of 0.9% sodium chloride solution for 8 weeks. After the experiment, the cardiac function, ET1 and AngⅡ content and pathological score in the serum were determined, and the pathological features of the myocardium were observed. Results: Left ventricular ejection fraction, interventricular septum thickness and left ventricular posterior wall thickness decreased, left ventricular end-diastolic diameter and left ventricular end-systolic diameter increased in Ginkgo flavonoids group and metoprolol group, which were significantly different from those in model group (P <0.05), indicating that after treatment, the heart function was improved. The content of ET-1, AngⅡ and the pathological score of total flavonoids of Ginkgo biloba decreased significantly compared with the model group (P <0.05). Conclusion: Ginkgo flavonoids can improve heart function in heart failure rats, its mechanism may be related to the reduction of ET-1, Ang Ⅱ content.