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为研究转EPSPS基因大豆NZL06-698在杂草环境中的生态适应性,试验设置杂草处理模拟野外环境,研究转基因大豆NZL06-698(GT698)的生存竞争能力以及外源EPSPS蛋白表达情况。结果表明:在相同处理下转基因大豆GT698的株高(R8期)、百粒重显著高于亲本大豆蒙豆12(MD12),但单株产量、单株籽粒数、结实率显著低于亲本大豆,说明外源基因的存在并未增强转基因大豆的生存竞争力,且转基因大豆在野外的生存竞争能力与亲本大豆相比较弱。试验注意到,杂草环境对转EPSPS基因大豆的生长有明显的限制作用,杂草处理下转基因大豆的单株产量、单株籽粒数、百粒重、结实率等指标均显著低于对照处理。ELISA检测结果表明,转基因大豆叶片及籽粒中外源EPSPS蛋白在杂草处理及对照中均正常表达,且两种处理下的EPSPS蛋白表达量无显著差异。以上结果表明杂草环境中转基因大豆的EPSPS蛋白正常表达,正常提供抗草甘膦性状,但是外源基因的存在并没有增加转基因大豆的生存竞争能力,且转基因大豆的生长受到杂草环境的显著抑制,由此得出结论:与亲本大豆相比,转EPSPS基因大豆NZL06-698在野外环境中生态适应能力较弱。
In order to study the ecological adaptability of EPSPS soybean NZL06-698 in weedy environment, the weed processing simulated field environment was set up to study the survival competition ability of transgenic soybean NZL06-698 (GT698) and the exogenous EPSPS protein expression. The results showed that the plant height (R8) and the 100-grain weight of the transgenic soybean GT698 were significantly higher than those of the parental soybean and mung bean 12 (MD12) under the same treatment, but the yield per plant, the number of grains per plant and the seed setting rate were significantly lower than those of the parent soybean , Indicating that the presence of foreign genes did not enhance the viability of transgenic soybean, and the viability of transgenic soybeans in the field was weaker than that of the parental soybean. The experiment noticed that the weed environment had a significant restriction on the growth of EPSPS-transgenic soybean. The single plant yield, grain number per plant, 100-grain weight and seed setting rate under weed treatment were significantly lower than the control . The results of ELISA showed that the EPSPS protein in transgenic soybean leaves and grains were normal expression in weed control and control, and there was no significant difference in EPSPS protein expression between the two treatments. The above results indicated that EPSPS protein of transgenic soybean was normally expressed in weed environment and normally provided glyphosate-resistant trait, but the presence of exogenous gene did not increase the viability of transgenic soybean, and the growth of transgenic soybean was significantly affected by weed environment Inhibition, which concluded that compared with the parental soybean, transgenic EPSSL soybean NZL06-698 had weaker ecological adaptability in the field.