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目的探讨肺动脉高压(pulmonary hypertension,PH)模型大鼠循环内皮祖细胞(endothelial progenitor cell,EPC)水平的变化及其与血浆一氧化氮(nitric oxide,NO)浓度的关系。方法利用野百合碱诱导大鼠发生肺动脉高压,使用流式细胞仪对其外周血CD45阴性、CD34/FLK-1双阳性的单个核细胞,以表示循环EPC,同时采用Greiss法对其血浆NO浓度进行检测。利用简单线性回归分析二者之间的关系。结果 PH模型组循环EPC水平明显低于对照组(0.016%±0.007%vs 0.031%±0.011%,t=3.144,P<0.01)。血浆NO浓度亦显著下降(19.66±2.78μmol/L vs 54.31±3.81μmol/L,t=20.784,P<0.01)。二者之间呈正相关(r=0.792,P<0.05)。结论 PH的发生可能与血浆NO浓度降低导致循环EPC水平下调有关。
Objective To investigate the changes of circulating endothelial progenitor cells (EPCs) and its relationship with plasma nitric oxide (NO) levels in pulmonary hypertension (PH) model rats. Methods Monocrotaline was used to induce pulmonary hypertension in rats. Flow cytometry was used to detect the expression of circulating circulating EPC in CD45-negative and CD34 / FLK-1 double positive mononuclear cells. Meanwhile, the plasma concentrations of NO were determined by Greiss method Test. Using simple linear regression analysis of the relationship between the two. Results The level of circulating EPC in PH model group was significantly lower than that in control group (0.016% ± 0.007% vs 0.031% ± 0.011%, t = 3.144, P <0.01). Plasma NO concentration was also significantly decreased (19.66 ± 2.78μmol / L vs 54.31 ± 3.81μmol / L, t = 20.784, P <0.01). There was a positive correlation between them (r = 0.792, P <0.05). Conclusions The occurrence of PH may be related to the decrease of circulating NO level and the decrease of circulating EPC levels.