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目的:通过对小鼠生长期实施运动干预,结束后去卵巢模拟妇女绝经后骨质疏松症,静养至晚年,研究生长期运动对去卵巢小鼠晚年骨密度、骨组织TGF-β1/Smad3信号通路的影响。方法:40只5周龄C57 BL/6雌性小鼠随机分为5组:安静组,运动组,假手术组,安静+去卵巢组,运动+去卵巢组。运动组和运动+去卵巢组进行下坡跑训练,每次40 min,每周训练5次,共训练8周。训练结束后进行第1次测试,取安静组和运动组两侧前肢检测骨密度;右侧胫骨和股骨提取mRNA,检测其骨组织TGF-β1、Smad3和OC的基因表达。同时假手术组实行去卵巢假手术,安静+去卵巢组和运动+去卵巢组摘除卵巢。此3组小鼠静养6周后处死,测试指标与第一次相同。结果:与安静组相比,运动组小鼠骨密度、骨组织TGF-β1、Smad3和OC的基因表达显著增加;与安静+去卵巢组相比,运动+去卵巢组小鼠骨密度、骨组织TGF-β1、Smad3和OC的基因表达显著增加。结论:生长期运动干预通过提高峰值骨量、促进小鼠骨组织成骨细胞的分化和活化,从而有效预防绝经期妇女骨质疏松症的发生;生长期运动干预对去卵巢小鼠成骨细胞分化和活化的促进作用可能与骨组织TGF-β1、Smad3和OC基因表达的变化有关。
OBJECTIVE: To study the effects of long-term exercise on bone mineral density, TGF-β1 / Smad3 signaling pathway in ovariectomized mice Impact. Methods: Forty five-week-old C57BL / 6 female mice were randomly divided into 5 groups: sedentary group, exercise group, sham operation group, sedentary + ovariectomized group and exercise + ovariectomized group. Exercise group and exercise + ovariectomized group were downhill training, each 40 min, training 5 times a week, a total of training for 8 weeks. The first test was conducted after training. The forelimbs of both sides of the quiet group and the exercise group were measured for bone mineral density. MRNA was extracted from the tibia and femur on the right to detect the gene expression of TGF-β1, Smad3 and OC. At the same time, the sham-operated group was given ovariectomized operation, and the sedentary + ovariectomized group and exercise + ovariectomized group were ovariectomized. The three groups of mice were sacrificed 6 weeks after sacrifice, the same indicators as the first test. Results: Compared with resting group, the BMD and the gene expression of TGF-β1, Smad3 and OC in exercise group were significantly increased. Compared with resting + ovariectomized group, the BMD, Tissue TGF-β1, Smad3 and OC gene expression increased significantly. Conclusion: During the growth period, the intervention of osteoporosis can promote the differentiation and activation of osteoblasts in the bone tissue of the mice by increasing the peak bone mass, which can effectively prevent the occurrence of osteoporosis in the menopausal women. During the growth phase, The promotion of differentiation and activation may be related to the changes of TGF-β1, Smad3 and OC gene expression in bone tissue.