论文部分内容阅读
目的观察并比较小鼠树突状细胞（ＤＣ）和巨噬细胞对外源性抗原的内吞路径的差异。方法制备小鼠骨髓ＤＣ和腹腔巨噬细胞，然后让ＤＣ和巨噬细胞与辣根过氧化物酶（ＨＲＰ）５ｎｍ胶体金共孵育１０分钟后再培养０～１２０分钟，进行酸性磷酸酶细胞化学反应和组织相容性抗原（ＭＨＣ）Ⅱ免疫胶体金标记，最后在电镜下观察５ｎｍ胶体金的细胞内运行路径。结果ＤＣ内吞ＨＲＰ５ｎｍ胶体金１０分钟后再培养３０分钟，大部分５ｎｍ胶体金进入ＭＨＣⅡ隔室（ＭＩＣ）中，只有极少数酸性磷酸酶阳性的溶酶体内含少量５ｎｍ胶体金。而与ＤＣ显著不同的是巨噬细胞摄入的大部分５ｎｍ胶体金进入了溶酶体，还有部分５ｎｍ胶体金进入ＭＩＣ中。内吞后再培养６０分钟，ＤＣ内仍有许多５ｎｍ胶体金，而巨噬细胞内已很少见到５ｎｍ胶体金。结论同一种外源性抗原被ＤＣ和巨噬细胞内吞后，在这两类细胞中的运行路径明显不同。内吞后３０分钟，巨噬细胞摄入的大部分抗原进入溶酶体，而ＤＣ则将大部分抗原送入ＭＩＣ，这可能与ＤＣ独特的抗原提呈功能有关。另外，巨噬细胞对抗原的清除能力比ＤＣ强得多。
Objective To observe and compare the difference of endonuclease pathways of exogenous antigens between dendritic cells (DCs) and macrophages in mice. Methods Mice bone marrow DCs and peritoneal macrophages were prepared, then DCs and macrophages were incubated with horseradish peroxidase (HRP) 5nm colloidal gold for 10 minutes and then cultured for 0 to 120 minutes to carry out acid phosphatase cells Chemical reaction and histocompatibility antigen (MHC) Ⅱ immunogold labeling. Finally, the intracellular path of 5nm colloidal gold was observed under electron microscope. Results DC endocytosed HRP 5nm colloidal gold 10 minutes and then cultured for 30 minutes, most of the 5nm colloidal gold into the MHC Ⅱ compartment (MIC), only a very small number of acid phosphatase-positive lysosomes containing a small amount of 5nm colloidal gold. The significant difference from DC is that most of the 5 nm colloidal gold taken up by macrophages enters the lysosome, and some 5 nm colloidal gold enters the MIC. After the endocytosis and then cultured for 60 minutes, there are still many 5nm colloidal gold in DC, while macrophages have rarely seen 5nm colloidal gold. Conclusion The same exogenous antigen was endocytosed by DCs and macrophages, and the pathways of operation in these two types of cells were significantly different. Thirty minutes after endocytosis, most of the macrophages ingested the lysosomes, while DCs sent most of the antigens into the MIC, which may be related to the unique antigen-presenting function of DCs. In addition, macrophages are much less able to clear antigens than DC.