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采用溶剂热法合成了共价有机框架材料,并使铂(Ⅳ)通过电化学还原沉积于此材料上,制成作为固定化基质的Pt/COF-LZUI。另据文献分别制备了MnO_2纳米材料,Pd NPs/MnO_2纳米复合材料,及以此为标记物的CRP抗体。用上述材料按规定程序修饰玻碳电极并制成夹心型CRP免疫传感器。利用固体核磁、X射线粉末衍射对COF-LZUI的结构和晶型结构进行了表征,利用透射电镜对COF-LZUl、Pt/COF-LZUl和Pd NPs/MnO_2纳米复合材料的形貌进行了表征。采用循环伏安法和计时电流法(i-t)研究该传感器的电化学特性及该电极对过氧化氢的电化学响应。该传感器的线性范围为1~150μg·L-1,检出限(3σ)为0.33μg·L~(-1)。
The covalent organic framework material was synthesized by solvothermal method, and Pt / COF-LZUI was immobilized on Pt (Ⅳ) as an immobilizing matrix by electrochemical reduction. According to the literature, MnO 2 nanomaterials, Pd NPs / MnO 2 nanocomposites and CRP antibodies were also prepared. Use the above materials modified glassy carbon electrode according to the prescribed procedures and made of sandwich-type CRP immunosensor. The structure and crystal structure of COF-LZUI were characterized by solid state nuclear magnetic resonance and X-ray powder diffraction. The morphology of COF-LZU1, Pt / COF-LZU1 and Pd NPs / MnO2 nanocomposites were characterized by transmission electron microscopy. The electrochemical properties of the sensor and the electrochemical response of the electrode to hydrogen peroxide were studied by cyclic voltammetry and chronoamperometry (i-t). The linear range of this sensor was 1 ~ 150μg · L-1, and the detection limit (3σ) was 0.33μg · L -1.