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目的:研究缬沙坦对动脉粥样硬化兔血清IL-8和TNF-α水平的影响。方法:将30只实验兔随机分为3组,每组10只,即正常对照组:喂以普通饲料;高脂饮食组:喂以高脂饮食(含15%蛋黄粉,0.5%胆固醇和5%猪油的饲料)6周,后给予10 ml/d生理盐水4周;药物干预组:喂以高脂饮食6周,后给予缬沙坦(10 mg/kg/d)治疗4周。饲养6周和10周时分别经兔耳缘静脉取血,通过酶联免疫法检测各组兔血清中IL-8和TNF-α的水平。结果:饲养第6周时,高脂饮食组和药物干预组兔血清TNF-α和IL-8水平均较正常对照组明显升高,差异均具有统计学意义(P<0.05),而高脂饮食组与药物干预组比较差异无统计学意义(P>0.05)。饲养第10周时,即缬沙坦干预4周后,药物干预组与建模6周时比较,血清TNF-α及IL-8水平均明显下降,差异具有统计学意义(P<0.05),且与高脂饮食组比较,血清TNF-α及IL-8水平亦明显下降,差异具有统计学意义(P<0.05)。结论:动脉粥样硬化时,血清IL-8和TNF-α升高,缬沙坦能明显降低动脉粥样硬化中IL-8和TNF-α水平,从而发挥抗动脉粥样硬化作用。
Objective: To investigate the effects of valsartan on serum IL-8 and TNF-α levels in atherosclerotic rabbits. Methods: Thirty rabbits were randomly divided into three groups (10 rats in each group): normal control group fed normal diet; high fat diet group fed with high fat diet (containing 15% egg yolk powder, 0.5% cholesterol and 5 % Lard for 6 weeks and then 10 ml / d saline for 4 weeks. The drug intervention group was fed with a high-fat diet for 6 weeks and then treated with valsartan (10 mg / kg / d) for 4 weeks. The rabbits were bled at 6 weeks and 10 weeks respectively. Serum levels of IL-8 and TNF-α were detected by enzyme-linked immunosorbent assay. Results: At the 6th week of feeding, the levels of TNF-α and IL-8 in the serum of the high-fat diet group and the drug-treated group were significantly higher than those of the normal control group (all P <0.05) There was no significant difference between diet group and drug intervention group (P> 0.05). After feeding for 10 weeks, that is, after 4 weeks of valsartan intervention, serum TNF-α and IL-8 levels were significantly decreased in the intervention group compared with the 6th week after modeling (P <0.05) Compared with the high-fat diet group, the levels of serum TNF-α and IL-8 also decreased significantly (P <0.05). CONCLUSIONS: Serum levels of IL-8 and TNF-α are elevated during atherosclerosis. Valsartan can significantly reduce the levels of IL-8 and TNF-α in atherosclerosis and thus play an anti-atherogenic role.