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建立一种敏感性高、特异性强的逆转录-聚合酶链反应(RT-PCR)方法,定量检测了30例原发性肝细胞癌(PHC)患者癌组织及癌周组织中多药耐药基因(mdrl)表达水平,其中5例患者术前经肝动脉插管化疗。结果显示:PHC癌组织中mdrl基因表达较癌周组织高;术前行化疗者癌组织中mdrl基因过度表达,同时其癌周组织中mdrl的表达较未化疗的癌周组织高。我们认为mdrl基因的过度表达在PHC内在性或获得性药物耐受的产生中起着一定的作用。
A high sensitivity and specificity reverse transcriptase polymerase chain reaction (RT-PCR) method was established to quantitatively detect multidrug resistance in cancer tissues and pericancerous tissues in 30 patients with primary hepatocellular carcinoma (PHC). The expression level of drug gene (mdrl), of which 5 patients were treated with hepatic artery catheterization before surgery. The results showed that the expression of mdrl gene in PHC cancer tissue was higher than that in pericancerous tissue. The mdrl gene was overexpressed in cancer tissue before operation, and the expression of mdrl in cancerous tissues was higher than that in non-chemotherapy. We believe that overexpression of the mdrl gene plays a role in the production of PHC intrinsic or acquired drug resistance.