论文部分内容阅读
目的:探讨嗜酸性粒细胞(EOS)对肺组织中的CD4+细胞分化的影响。方法:以鸡卵清蛋白(OVA)致敏和雾化吸入刺激BALB/c小鼠以诱发气道的EOS在气道聚集后将其分离纯化,然后与来源于致敏小鼠肺组织的CD4+细胞共同培养。在部分试验中加入抗CD80和(或)抗CD86mAb。同时,将EOS注入致敏小鼠气道,3d后,取出肺组织,并将其制成细胞悬液进行培养。收集培养上清液,用ELISA测定其中IL-4、IL-5、IL-13及INF-γ的含量。结果:在和EOS体外共同培养的条件下,肺组织CD4+淋巴细胞产生IL-4、IL-5、和IL-13等Th2类因子,但不产生INF-γ等Th1类因子;抗CD80和(或)抗CD86mAb可以明显抑制Th2类细胞因子的产生。将EOS注入致敏小鼠气道后,同样在体内能激发肺组织CD4+活化,产生Th2细胞因子IL-4,IL-5,IL-13,但不产生INF-γ,与体外结果相似,抗CD80和(或)抗CD86mAb可以抑制EOS在体内的抗原提呈过程。结论:EOS细胞在体内或体外均能摄取和处理抗原,激发CD4+的Th2反应。
Objective: To investigate the effect of eosinophils (EOS) on the differentiation of CD4 + cells in lung tissue. Methods: BALB / c mice were induced by inhalation of chicken ovalbumin (OVA) and aerosolized to induce airway EOS to be isolated and purified after airway aggregation, and then compared with CD4 + cells derived from sensitized mice lung Co-cultivation. In some experiments anti-CD80 and (or) anti-CD86 mAb were added. At the same time, EOS was injected into the airway of sensitized mice, and after 3 days, the lung tissues were removed and cultured in cell suspension. Culture supernatants were collected and assayed for IL-4, IL-5, IL-13 and INF-γ levels by ELISA. RESULTS: Th2 cytokines such as IL-4, IL-5 and IL-13 were produced by CD4 + lymphocytes in lung tissue under the conditions of co-culture with EOS, but not Th1-type factors such as INF- Or) anti-CD86 mAb can significantly inhibit the production of Th2 cytokines. Injecting EOS into the airway of sensitized mice also stimulates CD4 + activation in the lung tissue in vivo, resulting in Th2 cytokines IL-4, IL-5 and IL-13 but not INF-γ, similar to in vitro results CD80 and / or anti-CD86 mAb can inhibit the antigen-presenting process of EOS in vivo. CONCLUSIONS: EOS cells can both ingest and process antigens in vivo or in vitro, triggering CD4 + Th2 responses.