Nilsson等介绍了脾内免疫的方法。对此法灵敏度的估计表明当抗原固定于活化琼脂糖小珠时,总量约80毫微克的牛血清白蛋白(BSA)分散注于4点后能诱导产生血清抗体。微量抗原脾内免疫明显比腹腔内和静脉途径更易产生抗体。Spitz 等人报道了脾内一次注射20微克可溶性抗原或2.5×10~5个同种异体细胞均可产生抗体,此后又有所发展。 Nilsson et al introduced the method of spleen immunity. An estimate of the sensitivity of this method indicates that when the antigen is immobilized on activated sepharose beads, a total of about 80 nanograms of bovine serum albumin (BSA) dispersion is injected at 4 o’clock in order to induce the production of serum antibodies. Immunization of trace antigens in the spleen is significantly more likely to produce antibodies than intraperitoneal and intravenous routes. Spitz et al. Reported that an intraperitoneal injection of 20 micrograms of soluble antigen or 2.5 x 10 5 allogeneic cells produces antibodies and has since been developed.