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我们在开展农村结核病防治工作中,积极推广了痰液厚涂片检查法检查结核杆菌。这种检查方法,十多年来经我市郊区广大检验工作者试用,获得了较好效果,认为阳性检出率较高,方法简单,便于在农村推广。过去,我们采用的抗酸染色法,玻片既不能涂得过厚(过厚则镜检时光线不易透过)又不能涂得太薄(每毫升痰液中的结核杆菌少于5～10万时,则往往不易被查出),因此阳性检出率不高。为了克服这个缺点,我们改进了涂片和染色方法。我们选择门诊肺结核患者306人的痰标本,同时用齐-尼二氏抗酸染色法和厚涂片法检查结核杆菌,并进行了比较,结果发现厚涂片法比齐-尼二氏抗酸染色
In carrying out prevention and treatment of tuberculosis in rural areas, we actively promoted the examination of sputum smear thick test Mycobacterium tuberculosis. This method of inspection, more than 10 years by the majority of suburbs in our city test workers to try, get better results, that the positive detection rate is higher, the method is simple, easy to promote in rural areas. In the past, we used acid-fast staining, slides can not be coated too thick (too thick when the microscopic examination is not easy to pass the light) and can not be too thin (less than 5 ~ 10 Mycobacterium tuberculosis in sputum Million, it is often not easy to be detected), so the positive detection rate is not high. In order to overcome this disadvantage, we have improved smear and staining methods. We selected outpatient TB patients with sputum specimens of 306 patients, at the same time with Qi - Niacin acid-fast staining and thick smear method to check Mycobacterium tuberculosis, and were compared and found that the thick smear method Bisi - Nicotinic acid dyeing