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用 L BY- BX红细胞变形聚集仪检测 ED,采用 SDS-聚丙烯酰胺凝胶电泳分离红细胞膜蛋白并分析带3蛋白含量 ;取适量患者红细胞检测红细胞丙酮酸 - Cl- 交换速率常数。以红细胞 ATP和 2 ,3-二磷酸甘油酸 (2 ,3-DPG)含量作为衡量红细胞能量代谢状态的指标 ,观察 ATP和 2 ,3- DPG含量的变化 ,并与正常人比较。结果 :肝硬化患者 ED明显降低 ;凝胶电泳显示患者组带 3蛋白分布较窄 ,着色减弱 ,扫描图谱显示带 3蛋白的浓度峰明显低于对照组 ;患者组红细胞丙酮酸 - Cl- 交换速率常数明显低于对照组 ;肝硬化患者 ATP含量正常而 2 ,3- GPD含量明显增加。认为红细胞膜带 3蛋白含量减少及功能降低是肝硬化患者 ED降低的重要原因
ED was detected by L BY-BX erythrocyte deformable aggregator, and erythrocyte membrane protein was separated by SDS-polyacrylamide gel electrophoresis and the protein content of band 3 was measured. The appropriate amount of erythrocytes was used to detect erythrocyte pyruvate-Cl-exchange rate constant. The erythrocyte ATP and 2,3-diphosphoglycerate (2,3-DPG) content were used as indexes to measure the energy metabolism status of erythrocytes. The contents of ATP and 2, 3-DPG were observed and compared with normal subjects. Results: ED in patients with cirrhosis was significantly lower than that in control group. Gel electrophoresis showed that the distribution of band 3 protein was narrow and the coloration was weakened. The peak of band 3 protein in the group with liver cirrhosis was significantly lower than that in the control group. The erythrocyte pyruvate - Cl- exchange rate The constants were significantly lower than those in the control group. The content of ATP in patients with cirrhosis was normal and the content of 2, 3-GPD was significantly increased. It is considered that the decrease of erythrocyte membrane band 3 protein content and the decrease of function are the important reasons for the decrease of ED in cirrhotic patients