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原子力显微镜(AFM)能够以高分辨率和高灵敏度实现液体环境下活细胞的操作和检测,利用AFM力曲线测量功能还可以获得细胞力学特性的相关信息。在简要介绍AFM力曲线测量原理的基础上,从细胞的杨氏模量、硬度和配体-受体之间相互作用力三个方面,详细阐述了近年来AFM在细胞力学特性研究中的发展状况,并着重介绍了其在细胞杨氏模量研究中的进展,表明AFM已经成为细胞力学特性研究中重要的生物显微技术。最后,针对AFM的不足及局限性,提出了目前存在的问题,并对AFM在细胞力学特性研究中的前景进行了展望。
Atomic Force Microscopy (AFM) enables the manipulation and detection of living cells in liquid environment with high resolution and sensitivity. The AFM Force Curve Measurement also provides information on the cellular mechanics properties. Based on the brief introduction of the principle of AFM force curve measurement, the development of AFM in the study of cellular mechanical properties was expounded in detail from three aspects of Young’s modulus, hardness and the interaction between ligand and receptor. The progress of the study on the Young’s modulus of cells has been highlighted. It indicates that AFM has become an important biological microscopy in the study of cellular mechanical properties. Finally, in view of the shortcomings and limitations of AFM, the existing problems are put forward and the prospect of AFM in the study of cellular mechanical properties is prospected.