论文部分内容阅读
目的 探讨半胱氨酸蛋白酶caspase 3在 β淀粉样肽1 4 0 (β AP1 4 0 )诱导大鼠皮层神经元凋亡中的可能作用。 方法 用 β AP1 4 0 诱导神经元凋亡 ,同时检测caspase 3活力和caspase 3活性片段及caspase 3mRNA的表达水平。 结果 4 0mg·L- 1 的凝聚态 β AP1 4 0 诱导大鼠皮层神经元凋亡过程中 ,caspase 3活力和caspase 3mRNA的表达水平均有明显增高 (P <0 0 1) ;特异性的caspase 3抑制剂Ac DEVD CHO对caspase 3的激活和皮层神经元细胞凋亡均有明显的阻断作用。 结论 caspase 3可能是 β AP1 4 0 诱导大鼠皮层神经元凋亡的效应因子
Objective To investigate the possible role of caspase 3 in the apoptosis of rat cortical neurons induced by β amyloid peptide 140 (β AP1 40). Methods The apoptosis of neurons was induced by β AP1 40 and the activity of caspase 3 and the activity of caspase 3 and caspase 3 mRNA were detected. Results The caspase 3 activity and the expression of caspase 3 mRNA were significantly increased (P <0.01) in the 40 mg · L-1 condensed β AP1 40 induced cortical neuron apoptosis. The specific caspase 3 inhibitor Ac DEVD CHO can obviously block the activation of caspase 3 and the apoptosis of cortical neurons. Conclusions Caspase 3 may be the effector of β AP1 4 0 induced apoptosis in rat cortical neurons