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目的 探讨不同慢性肝病患者血清HBVDNA水平与病变程度关系 ;肝癌患者血清HBVDNA水平与HBeAg的关系。方法 应用定量聚合酶链反应法检测 6 7例慢性乙型肝炎、4 5例肝炎肝硬化、5 2例肝癌患者血清HBVDNA水平 ,应用酶联免疫吸附法检测肝癌患者血清乙型肝炎病毒标志 (HBVM)。结果 血清HBVDNA水平 :肝硬化、肝癌患者均显著高于慢性乙肝患者 (P <0 .0 5 ) ;肝癌患者中HBsAg(+)、HBeAg(+)组血清HBVDNA水平显著高于HBsAg(+)、抗 HBe(+)组 (P <0 .0 5 )。结论 与乙肝相关的肝硬化、肝癌存在HBV高水平复制 ,部分抗 HBe(+)者HBV复制水平仍较高
Objective To investigate the relationship between serum HBVDNA level and the degree of disease in patients with chronic liver disease and the relationship between serum HBVDNA level and HBeAg in patients with liver cancer. Methods The level of serum HBV DNA in 67 patients with chronic hepatitis B, 45 patients with hepatic cirrhosis and 52 patients with hepatocellular carcinoma was detected by quantitative polymerase chain reaction. The serum hepatitis B virus (HBVM) was detected by enzyme-linked immunosorbent assay (ELISA) ). Results Serum HBVDNA levels in patients with liver cirrhosis and liver cancer were significantly higher than those in patients with chronic hepatitis B (P <0.05). Serum HBVDNA levels in HBsAg (+) and HBeAg (+) patients were significantly higher than those in HBsAg (+), Anti-HBe (+) group (P <0.05). Conclusions There is a high level of HBV replication in cirrhosis and hepatocellular carcinoma associated with hepatitis B, and the level of HBV replication in some anti-HBe (+) patients is still high