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目的:研究红景天有效成分对缺氧缺糖心肌细胞损的保护作用。方法:建立体外缺氧缺糖心肌细胞损伤模型,分别测定细胞MTT、LDH和MDA的变化,观察红景天单体成分红景天苷、酪醇、没食子酸、德钦红景天苷、草质素-7-O-(3’-β-D-葡萄糖)-α-L-鼠李糖苷5种成分对心肌细胞损伤的保护作用,并运用反转录聚合酶链式反应(RT-PCR)法检测不同单体成分对心肌细胞HIF-1αmRNA的表达。结果:5种单体成分均有不同程度的促细胞活力,减少LDA和MDA的含量,其中单体成分红景天苷、酪醇能明显上调HIF-1αmRNA的表达。结论:红景天有明显抗缺氧作用,5种单体成分对缺氧缺糖心肌细胞损伤具有明显的保护作用,其机制与上调HIF-1αmRNA的表达相关。
Objective: To study the protective effect of active ingredients of Rhodiola on myocardial damage induced by hypoxia and glucose deprivation. METHODS: A model of myocardial injury induced by hypoxia and glucose deprivation in vitro was established. The changes of MTT, LDH and MDA in cells were determined. The components of Rhodiola rosea, tyrosol, gallic acid, deqin, and salidroside were observed. Protection of Myocardial Cell Injury by Five Components of 7-O-(3’-β-D-Glucose)-α-L-Rhamnoside and the Application of Reverse Transcription Polymerase Chain Reaction (RT-PCR) The method used to detect the expression of HIF-1α mRNA in cardiomyocytes by different monomer components. RESULTS: The five monomer components all had different degrees of promoting cell viability and decreased the contents of LDA and MDA. The monomeric components salidroside and tyrosol significantly increased the expression of HIF-1α mRNA. Conclusion: Rhodiola has obvious anti-hypoxia effect. The five kinds of monomer components have obvious protective effects on myocardial injury induced by hypoxia and glucose deprivation, and its mechanism is related to up-regulation of HIF-1αmRNA expression.