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目的探究蓖麻连作对根际微生物多样性的影响。方法以两年连作蓖麻根际土壤(蓖2)、一年生蓖麻根际土壤(蓖1)和相邻的未种植过蓖麻的大麦根际土壤(大麦)为研究对象,利用Biolog技术对样品进行检测,通过SPSS数据分析软件对测得的数据进行分析。结果随着培养时间的延长,平均每孔颜色变化率(AWCD)值不断增加,在72~108h变化最快,在120h后,蓖1与蓖2的AWCD值均趋向平稳;香浓指数的比较说明各个样品之间差异存在统计学意义(P<0.05);随着培养时间的延长,不同样品利用碳源的能力为:蓖1>蓖2>大麦;主成分分析显示蓖1根际微生物多样性最强,蓖2次之,大麦根际微生物多样性最差。结论本研究所得结果为提高蓖麻的质量与产量提供理论依据。
Objective To investigate the effects of continuous castor cultivation on rhizosphere microbial diversity. Methods Two-year continuous castor rhizosphere soil (grate 2), annual castor rhizosphere soil (grate 1) and adjacent barley rhizosphere soil (barley) were planted with Biolog technology Samples were tested by SPSS data analysis software to analyze the measured data. Results As the incubation time prolonged, the average color change rate per hole (AWCD) increased steadily from 72h to 108h, and AWCD values tended to be stable after 120h. The comparison of sweetness index (P <0.05). With the prolongation of culture time, the ability of different samples to utilize carbon source was: gh1> gh2> barley. The principal component analysis showed that rhizosphere microbes The strongest, grate 2 times, barley rhizosphere microbial diversity is the worst. Conclusion The results obtained in this study provide a theoretical basis for improving the quality and yield of castor.