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目的:研究99mTc标记的抗心肌肌钙蛋白Ⅰ单克隆抗体(AcTnIMA)在实验性急性心肌损伤大鼠体内的分布,探讨99mTc-AcTnIMA是否可以作为心脏放射免疫显像剂。方法:实验组:20只急性心肌损伤大鼠注射99mTc-AcTnIMA0.2mci,分别于注射后2、4、6、8h处死(每次5只),取血液、肝、脾、肾、正常肌肉、肺和心脏,以计算每克组织放射性计数占总注入计数的百分比(ID%/g)及心-肺ID%/g比(HLR)。药物对照组:20只急性心肌损伤大鼠注射99mTc标记的非特异性免疫球蛋白(N-IgG)0.2mci,处死方法同实验组。取肺及心脏,计算ID%/g及HLR;空白对照组:20只正常大鼠注射99mTc-AcTnIMA0.2mci,处理方法同药物对照组。结果:急性损伤心肌对99mTc-AcTnIMA为特异性摄取,摄取的高峰时间为4h。结论:99mTc-AcTnIMA有望作为心脏放射免疫显像剂诊断急性心肌损伤。
Objective: To investigate the distribution of 99mTc-labeled anti-cardiac troponin I monoclonal antibody (AcTnIMA) in rats with acute myocardial injury and to investigate whether 99mTc-AcTnIMA can be used as a radioimmunoimaging agent for cardiac injury. Methods: The experimental group: 20 acute myocardial injury rats injected with 99mTc-AcTnIMA0.2mci were killed at 2h, 4h, 6h and 8h after injection respectively (5 rats each). Blood, liver, spleen and kidney, normal muscle, Lung and heart to calculate the percentage of radioactive counts per gram of tissue (ID% / g) and heart-lung ID% / g ratio (HLR). Drug control group: 20 acute myocardial injury rats were injected with 99mTc labeled non-specific immunoglobulin (N-IgG) 0.2mci, the same method as the experimental group. The lungs and heart were taken and ID% / g and HLR were calculated. Blank control group: 20 normal rats were injected with 0.2mci of 99mTc-AcTnIMA, and the treatment method was the same as that of the drug control group. Results: 99mTc-AcTnIMA was specifically up-regulated in acute myocardium, and the peak time was 4h. Conclusion: 99mTc-AcTnIMA is expected to be used as a cardiac radioimmunoimaging agent in the diagnosis of acute myocardial injury.