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目的:建立高效液相色谱法同时测定不同采收期荭草中荭草素、异荭草素含量的方法。方法:色谱柱为AgilentC18(4.6 mm×150 mm,5μm),流动相乙腈-0.1%磷酸水梯度洗脱,检测波长350 nm,流速1.0 mL.min-1。结果:荭草素在0.068 2～0.682μg(r=0.999 7)线性关系良好,平均回收率为99.94%,RSD 1.08%;异荭草素在0.386～3.860μg(r=0.999 3)线性关系良好,平均回收率为99.67%,RSD 1.09%。结论:方法简便、快捷、准确的特点,可用于荭草药材的质量控制。
Objective: To establish a method for the simultaneous determination of tenardin and isohumulone in Achnatherum septenycetes collected by high performance liquid chromatography. Methods: The column was Agilent C18 (4.6 mm × 150 mm, 5 μm). The mobile phase was eluted with a gradient of acetonitrile-0.1% phosphoric acid. The detection wavelength was 350 nm and the flow rate was 1.0 mL.min-1. Results: The calibration curve was linear over the range of 0.068 2 ~ 0.682 μg (r = 0.999 7) with an average recovery of 99.94% and RSD 1.08%. The isoenzyme was linear at 0.386 ~ 3.860 μg (r = 0.999 3) , The average recovery was 99.67%, RSD 1.09%. Conclusion: The method is simple, rapid and accurate. It can be used for the quality control of Potamogeton crispus.