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目的评价每周小剂量重复照射大鼠放射性肺损伤模型的可行性、科学性及实用性。方法36只Wistar种雌性大鼠,按5:1比例随机分为照射组(A组)30只、对照组(B组)6只。6-MV直线加速器对A组大鼠右肺进行分次照射(5Gy/次,1次/周,累积剂量最高为30Gy),于照射后第4、6、8、12、26周处死,每时相点处死6只,B组于第4周末处死。观察动物活体外观、肺的大体标本、行HE染色观察大鼠肺组织学变化、ELISA法检测血清和SP法检测肺组织中的TGF-β1蛋白表达。结果A组受照肺组织表现出放射性肺炎及肺纤维化,大鼠血清和肺组织TGF-β1表达第4周表达升高,到第12周达到高峰,26周下降。结论每周小剂量重复照射所制作的大鼠放射性肺损伤模型稳定可靠,动物死亡率低,符合临床放疗实际。
Objective To evaluate the feasibility, scientificalness and practicability of weekly low-dose repeated radiation radiation-induced lung injury in rats. Methods Thirty - six Wistar female rats were randomly divided into irradiation group (A group) 30 and control group (B group) 6 according to the ratio of 5: 1. 6-MV linear accelerator on the right lung of rats in group A (5Gy / time, 1 time / week, the cumulative dose of up to 30Gy), were killed at 4,6,8,12,26 weeks after irradiation, each Six rats were sacrificed at the time point, Group B was sacrificed at the end of the fourth week. The appearance of animals and the gross specimens of the lungs were observed. The histopathological changes of the lungs were observed by HE staining. The expression of TGF-β1 protein in lung tissues was detected by ELISA and SP method. Results Group A showed radiation pneumonitis and pulmonary fibrosis in irradiated lung tissue. The expression of TGF-β1 in serum and lung tissue of rats in group A increased at the 4th week, reached its peak at 12th week, and decreased at 26 weeks. Conclusion The model of radiation-induced lung injury made by small dose of repeated weekly irradiation is stable and reliable, animal mortality is low, in line with the actual clinical radiotherapy.