以带有玉米褪绿斑驳病毒(MCMV)的玉米叶片为材料,研究建立了MCMV的普通RT-PCR、TaqMan实时荧光RTPCR和SYBR GreenⅠ实时荧光RT-PCR检测方法,并比较了3种方法的灵敏度。结果表明:TaqMan实时荧光RT-PCR的检测灵敏度最高,最低检出量可达1.61 fg,SYBR GreenⅠ实时荧光RT-PCR略逊之,普通RT-PCR的检测灵敏度则相对较低,与前两者相差10~100倍。 In this study, MCMV common RT-PCR, TaqMan real-time fluorescence RTPCR and SYBR GreenⅠ real-time fluorescence RT-PCR method were used to study maize leaves with maize chlorotic mottle virus (MCMV). The sensitivity of the three methods was compared . The results showed that the detection sensitivity of TaqMan real-time RT-PCR was the highest, the lowest detectable amount was 1.61 fg, the real-time fluorescence RT-PCR of SYBR GreenⅠ was slightly less, and the detection sensitivity of ordinary RT-PCR was lower, A difference of 10 to 100 times.