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为了正确检测和研究高频电刺激(high frequencystimulation,HFS)期间神经元的动作电位发放活动,进而深入揭示深部脑刺激治疗神经系统疾病的机制,本课题研究HFS期间锋电位波形的变化.在麻醉大鼠海马CA1区的输入神经通路Schaffer侧支上,施加1~2 min时长的100或者200 Hz顺向高频刺激(orthodromic-HFS,O-HFS),利用微电极阵列采集刺激下游神经元的多通道锋电位信号,并获得由O-HFS经过单突触传导激活的中间神经元的单元锋电位波形及其特征参数.结果表明,O-HFS使得锋电位的幅值明显减小而半高宽明显增加,以基线记录为基准计算百分比值,O-HFS期间锋电位的降支幅值和升支幅值分别可减小20%和40%左右,半高宽则增加10%以上.并且,在大量神经元同时产生动作电位期间,或者在比200 Hz具有更大兴奋作用的100 Hz刺激期间,锋电位波形的改变更多,幅值的减小可达50%,宽度的增加可达20%.可以推测,高频电刺激对于神经元的兴奋作用可能升高细胞膜电位,从而改变细胞膜离子通道的活动特性,导致动作电位波形的改变.这些结果支持深部脑刺激具有兴奋性调节作用的假说,对于正确分析高频电刺激期间神经元锋电位活动具有指导意义,也为进一步研究深部脑刺激(DBS)治疗脑神经系统疾病的机制提供了重要线索.
In order to correctly detect and study the action potential distribution of neurons during high frequency electrical stimulation (HFS) and further reveal the mechanism of deep brain stimulation in the treatment of neurological diseases, this study investigated the changes of the frontal waveforms during HFS, Rat hippocampal CA1 region input Schaffer collateral channels, the application of 1 ~ 2 min length of 100 or 200 Hz forward high-frequency stimulation (orthodromic-HFS, O-HFS), the use of micro-electrode array to stimulate the downstream neurons Multi-channel frontal potential signal and obtain the unit front potential waveform and its characteristic parameters of the interneurons activated by O-HFS through single synaptic conduction.The results show that O-HFS makes the amplitude of the frontal potential decrease obviously and the half height Width and base line, the descending and ascending amplitudes of the front potential during O-HFS can be reduced by about 20% and 40%, respectively, and the FWHM increases by more than 10%. During the simultaneous generation of action potentials by a large number of neurons, or at 100 Hz stimulation, which is more exciting than 200 Hz, the change of the frontal potential waveform is more and the amplitude is reduced by up to 50%, with an increase in width up to 20%. Can push The excitatory effects of high-frequency electrical stimulation on neurons may increase the cell membrane potential and thus change the activity characteristics of ion channels in the cell membrane, leading to changes in the action potential waveform.These results support the hypothesis that excitatory regulation of deep brain stimulation has a positive effect on the correct analysis Neuronal spike activity during high-frequency electrical stimulation is instructive and provides important clues to further study the mechanism of deep brain stimulation (DBS) in the treatment of neurological diseases of the brain.