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目的 探讨移植血红素氧合酶-1(Heme Oxygenase,HO-1)修饰的骨髓间充质干细胞(mes-enchymal stem cells,MSCs)对大鼠梗死心肌炎症细胞因子及心室重构的影响.方法 取大鼠骨髓,扩增培养MSCs;HO-1或GFP腺病毒转染、DAPI标记MSCs.结扎大鼠左前降支制造心肌梗死模型1 h后,分别将HO-1-MSCe、GFP-MSCs、PBS注射到大鼠心脏梗死区边缘,移植后第4天,Western Blot检测HO-1蛋白在梗死区边缘的表达,ELISA检测VEGF、bFGF、HGF的表达,RT-PCR检测炎症细胞因子TNF-α、IL-1β、IL-6、IL-10 mRNA的表达.超声心动图检测大鼠心功能变化.移植4周后,梗死区心肌HE,Masson染色检测左室重构.结果 HO-1-MSCs移植组与GFP-MSCs、PBS移植组相比,HO-1蛋白在梗死区边缘稳定高效表达;细胞因子VEGF、bFGF、HGF表达增加(P<0.05);炎症细胞因子TNF-α、IL-1β、IL-6 mRNA表达减少(P<0.05),而IL-10 mRNA表达增加(P<0.05);左室舒张末期和收缩末期内径缩短,左室前壁增厚,心室腔减小,梗死区血管密度上调,胶原形成明显减少.结论 HO-1-MSCS分泌多种生物活性细胞因子,协同HO-1蛋白改善炎症微环境,促进血管生成,减少胶原形成,从而延缓梗死区心窒重构,有效改善心功能.“,”Objective To investigate the effects of mesenchymul stem cells(MSCs) transfected with human home oxygenase- 1 gene on the inflammatory cytokines and the ventricular remodeling after myocardial infarction in rats.Method MSCs were acquired from the hone marrow of adults rats.They were isolated,purified cultured,and transfected with Adv-HO-1,or Adv-GFP in vitro before transplantation.At 1 hours after left coronary artery ligation,Adv-HO-1-MSCs or Adv-GFP-MSCs marked with DAPI were directly injected into the horder of cardiac infarction in rats.At 4 days after transplantation,western blot analysis was used to measure HO-1 protein expression in the the horder of cardiac infarction.The levels of VEGF,bFGF,HGF protein expression were measured by ELISA,and the levels of TNF-α,IL-1β,IL-6,IL-10 mRNA expression were measured by RT-PCR.The rat heart function was measured by echocardiography.At 4 weeks after transplantation,ventricular remodeling and pathological changes were measured by HE and Masson staining.Results The Adv-HO-1-MSCa treated group showed marked increase of HO-1 rotein (P<0.05),and displayed significant increase of montioned cytokines above,P <0.05,compared with other groups.The Adv-HO-1-MSCs treated group displayed significant reduction of mRNAs expreesion of TNF-α,IL-1β,IL-6,and significant increase in IL-10 mRNA expression,with P<0.05,compared with others.Conclusions HO-1-MSCs could secrete multiple cytokines in infarction hearts,and had beneficial effects on inflammatory cytokines,remodeling processes and cardiac function.